CELLULAR PATHWAYS INVOLVED IN VIRAL INDUCTION--THEIR ROLE IN HIV PATHOGENESIS

参与病毒诱导的细胞途径——它们在 HIV 发病机制中的作用

• 批准号: 3770409
• 负责人: I K HEWLETT
• 金额: --
• 依托单位: BUREAU OF HEALTH PLANNING AND RESOURCES DEVELOPMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3770409
• 关键词: AIDS; aflatoxins; benzopyrenes; biological signal transduction; cell bank /registry; cell population study; chemical carcinogen; cytokine; disease /disorder proneness /risk; environmental toxicology; human tissue; latent virus infection; mutagens; nitrosamines; second messengers; tumor necrosis factor alpha; virus antigen; virus replication

Induction of HIV from a latent state was studied using the U1 cell line. Specifically, the effect of various chemical carcinogens and environmental mutagens was examined for their ability to induce virus from this cell line with the objective of identifying risk factors of physiologic relevance that may affect disease progression in AIDS. U1 cells were treated with 2 different concentrations of various carcinogens. Virus production was measured by p24 antigen, RT, DNA and RNA analysis. Enhances virus production was seen in cells treated with benzopyrene, NNK and aflatoxin B. Viral induction was accompanied by an increase in NF-KB activity and production of cytokines such as TNF-alpha. In the case of aflatoxin, virus induction appeared to be a TNF-a independent mechanism. The role of other cytokines, second messengers and cellular signaling pathways in the induction of HIV by these agents is currently under investigation. These experiments are also being performed in chronically infected cells that produce low levels of virus.

使用U1细胞系研究了HIV从潜伏状态的诱导。 具体来说，各种化学致癌物和环境的影响， 检测诱变剂从该细胞系诱导病毒的能力 目的是确定与生理相关的风险因素 这可能会影响艾滋病的病情发展。 U1细胞用2 不同浓度的致癌物质。 病毒的产生是 通过p24抗原、RT、DNA和RNA分析测定。增强病毒 在用苯并芘、NNK和黄曲霉毒素B处理的细胞中观察到产生。 病毒诱导伴随着NF-κ B活性的增加， 产生细胞因子如TNF-α。 就黄曲霉毒素而言， 诱导似乎是TNF-α独立的机制。 其他人的作用 细胞因子、第二信使和细胞信号通路 目前正在研究这些药剂对HIV的诱导作用。 这些 实验也在慢性感染的细胞中进行， 产生低水平的病毒。