INFECTION OF NON-HEMATOPOIETIC CELLS WITH HIV-1 AND HIV-2

已知HIV-1可感染多种非淋巴细胞系， 如果入境方式被推定为涉及额外的或 一种独立的受体或第二信使样分子。广度 在这些细胞系中病毒复制的情况与 产生性至流产感染提示宿主依赖控制 关于病毒复制的研究。这些模型系统是识别的理想选择 与再感染有关的细胞和病毒因素。此外，在某些情况下 例如，来自患者的某些上皮细胞(例如结肠、宫颈 等)已被证明含有病毒核酸。最新流行病学 研究表明，某些传播给胎儿的途径可能发生在子宫内。 可能涉及非淋巴细胞类型的感染。我们已经发起了 探索活动性或可能流产病毒机制的研究 这些细胞类型中的感染。我们选择的工作目标单元 人胚胎神经母细胞瘤细胞系SKNSH、SKNMC L132，两株宫颈癌细胞系SIHA和C33A，两株子宫细胞 细胞系HS 825T和HS 258T，阴道癌细胞系HS 769 VG。这些细胞 LINE已经感染了HIV-1，感染过程正在 被监视着。病毒复制动力学的评价--合成 病毒DNA、RNA和蛋白质正在这些细胞系中进行分析。 细胞因子如NFkB、NFAT、c-fos/jun、激酶、甲基酶和 细胞因子可能控制病毒的不同程度 将对这些不同的细胞系中发生的复制进行研究。

HIV-1 has been known to infect a variety of non lymphoid cell lines, where the mode of entry has been postulated to involve an additional or an independant receptor or a second messenger-like molecule. The extent of viral replication in these cell lines is vastly different from productive to abortive infections suggestive of host dependant controls on viral replication. These model systems are ideal for identification of cellular and viral factors involved in relication. Also, in some instances, certain epithelial cells from patients ( e.g colon, cervical etc) have been shown to harbor viral nucleic acid. Recent epidemiologic studies suggest that some transmission to the fetus may occur in utero and may involve infection of non lymphoid cell types. We have initiated studies to explore mechanisms of active or possibly abortive viral infections in these cell types. The target cells we have chosen to work with are neuroblastoma cell lines SKNSH, SKNMC, a human embryonic cell line, L132, two cervical cell lines SiHa and C33A, two uterine cell lines HS 825T and HS 258T and a vaginal cell line HS 769 Vg. These cell lines have been infected with HIV-1 and the course of infection is being monitored. The kinetics of viral replication assessed by synthesis of viral DNA, RNA and proteins is being analyzed in these cell lines. Cellular factors such as NFkB, NFAT, c-fos/jun, kinases, methylases and cytokines that may govern the different extents to which viral replication occurs in these different cell lines will be investigated.