INFECTION OF NON-HEMATOPOIETIC CELLS WITH HIV-1 AND HIV-2

HIV-1 和 HIV-2 感染非造血细胞

• 批准号: 3792570
• 负责人: I K HEWLETT
• 金额: --
• 依托单位: CENTER BIOLOGICS EVALUATION RESEARCH TRANSFUSION
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3792570
• 关键词: DNA binding protein; HIV infections; cytokine; epithelium; human immunodeficiency virus 1; human immunodeficiency virus 2; methyltransferase; nucleic acid biosynthesis; oncogenes; protein biosynthesis; protein kinase; tissue /cell culture; virus infection mechanism; virus replication

HIV-1 has been known to infect a variety of non lymphoid cell lines, where the mode of entry has been postulated to involve an additional or an independant receptor or a second messenger-like molecule. The extent of viral replication in these cell lines is vastly different from productive to abortive infections suggestive of host dependant controls on viral replication. These model systems are ideal for identification of cellular and viral factors involved in relication. Also, in some instances, certain epithelial cells from patients ( e.g colon, cervical etc) have been shown to harbor viral nucleic acid. Recent epidemiologic studies suggest that some transmission to the fetus may occur in utero and may involve infection of non lymphoid cell types. We have initiated studies to explore mechanisms of active or possibly abortive viral infections in these cell types. The target cells we have chosen to work with are neuroblastoma cell lines SKNSH, SKNMC, a human embryonic cell line, L132, two cervical cell lines SiHa and C33A, two uterine cell lines HS 825T and HS 258T and a vaginal cell line HS 769 Vg. These cell lines have been infected with HIV-1 and the course of infection is being monitored. The kinetics of viral replication assessed by synthesis of viral DNA, RNA and proteins is being analyzed in these cell lines. Cellular factors such as NFkB, NFAT, c-fos/jun, kinases, methylases and cytokines that may govern the different extents to which viral replication occurs in these different cell lines will be investigated.

已知HIV-1感染多种非淋巴细胞系， 如果进入的方式被假定涉及额外的或 独立受体或第二信使样分子。程度 病毒在这些细胞系中复制的过程与 提示宿主依赖性控制的生产性至流产性感染 关于病毒复制。这些模型系统是理想的识别 细胞和病毒因子参与的关系。 此外，在一些 在某些情况下，来自患者的某些上皮细胞（例如结肠、宫颈 等）已显示含有病毒核酸。 最近的流行病 研究表明，一些传播给胎儿可能发生在子宫内， 并且可能涉及非淋巴样细胞类型的感染。 我们已开始 研究探讨活跃或可能流产的病毒的机制 感染这些细胞类型。 我们选择的靶细胞 使用的是神经母细胞瘤细胞系SKNSH、SKNMC、人胚胎细胞 细胞系，L132，两个宫颈细胞系SiHa和C33 A，两个子宫细胞系 细胞系HS 825 T和HS 258 T以及阴道细胞系HS 769 Vg。这些细胞 已经感染了HIV-1，感染过程正在 监测。 病毒复制的动力学通过合成 在这些细胞系中分析病毒DNA、RNA和蛋白质。 细胞因子如NFkB、NFAT、c-fos/jun、激酶、甲基化酶和 细胞因子可能决定了病毒感染的不同程度， 将研究在这些不同细胞系中发生的复制。