EFFICACY OF PASSIVE IMMUNOTHERAPY IN AIDS ASSESSED BY PCR

通过 PCR 评估被动免疫疗法治疗艾滋病的疗效

• 批准号: 3804851
• 负责人: I K HEWLETT
• 金额: --
• 依托单位: CENTER BIOLOGICS EVALUATION RESEARCH TRANSFUSION
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3804851
• 关键词: AIDS; HIV infections; blood transfusion; human subject; human therapy evaluation; human tissue; immunoglobulins; immunotherapy; longitudinal human study; neutralizing antibody; passive immunization; plasma; polymerase chain reaction; virus DNA; virus RNA; virus antigen

A hyperimmune plasma collected from healthy, HIV-1 infected individuals with high titers of neutralizing antibodies was administered to 7 AIDS and 3 ARC patients. 500 ml of plasma was administered monthly for 10 months. All patients were monitored for viral antibodies, antigen and ciral nucleic acid by PCR. DNA and RNA-PCR was performed on nucleic acid isolated from serum using primers from the gag region of the HIV-1 genome. HIV-1 DNA and RNA were detected prior to administration of immunoglobulin in all patients. No viral nucleic acid could be detected in serum after the first transfusion in any patient and this remained so for the length of the study in ARC patients. Viral sequences were detected in 3/7 AIDS patients towards the end of the trial (10-11 months). HIV antigen was not detected, except in 2 AIDS patients towards the end of the trial. These results indicate that hyperimmune plasma eliminated detection of HIV nucleic acid from the plasma of both AIDS and ARC patients although the effect was not as sustained in AIDS. Passive immunization may be useful in clearing plasma viremia, particularly in ARC patients. In a prospective study, serum and cells from 50 patients enrolled in a controlled study for evaluation of the therapeutic value of hyperimmune plasma, are being analyzed by PCR for HIV DNA and RNA.

从健康的HIV-1感染者采集的高免血浆 用高滴度中和抗体对7例艾滋病人和 3例ARC患者。每月给药500毫升，连续10个月。 所有患者都接受了病毒抗体、抗原和环核的监测。 用聚合酶链式反应检测酸度。DNA和RNA-聚合酶链式反应(RNA-聚合酶链式反应) 使用来自HIV-1基因组Gag区域的引物的血清。HIV-1DNA和 在注射免疫球蛋白之前对所有患者进行了RNA检测 病人。第一次检测后，血清中未检测到病毒核酸 在所有患者中都进行了输血，而且在研究期间一直如此 在ARC患者中。在7名艾滋病患者中检测到3/7的病毒序列 接近试验结束时(10-11个月)。没有检测到HIV抗原， 除了2名艾滋病患者在试验接近尾声时。这些结果 说明高免血浆消除了HIV核酸的检测 来自艾滋病和ARC患者的血浆，尽管效果不是 在艾滋病中持续存在。被动免疫可能有助于清除 血浆病毒血症，尤其是ARC患者。在一项前瞻性研究中， 参加一项对照研究的50名患者的血清和细胞 对高免疫血浆治疗价值的评估，正在进行中 用聚合酶链式反应分析HIV DNA和RNA。