EFFICACY OF PASSIVE IMMUNOTHERAPY IN AIDS ASSESSED BY PCR

从健康的HIV-1感染者中采集的超免疫血浆 用高滴度的中和抗体对7名艾滋病患者进行了治疗， 3例ARC患者。 每月给予500 ml血浆，持续10个月。 对所有患者进行病毒抗体、抗原和西拉尔核酸监测 酸通过PCR。 DNA和RNA-PCR对从大肠杆菌中分离的核酸进行。 使用来自HIV-1基因组的gag区域的引物， HIV-1 DNA和 所有受试者在免疫球蛋白给药前均检测到RNA。 患者 第一次接种后，血清中未检测到病毒核酸。 任何患者的输血，并在研究期间保持不变 在ARC患者中。 3/7例AIDS患者检出病毒序列 试验结束时（10-11个月）。 未检出HIV抗原， 除了在试验结束时的2名艾滋病患者。 这些结果 表明超免疫血浆消除了HIV核酸检测 从艾滋病和ARC患者的血浆中， 在艾滋病中持续存在。 被动免疫可能有助于清除 血浆病毒血症，特别是ARC患者。 在一项前瞻性研究中， 来自50名参加对照研究的患者的血清和细胞， 超免疫血浆的治疗价值的评价，正在 通过PCR分析HIV DNA和RNA。

A hyperimmune plasma collected from healthy, HIV-1 infected individuals with high titers of neutralizing antibodies was administered to 7 AIDS and 3 ARC patients. 500 ml of plasma was administered monthly for 10 months. All patients were monitored for viral antibodies, antigen and ciral nucleic acid by PCR. DNA and RNA-PCR was performed on nucleic acid isolated from serum using primers from the gag region of the HIV-1 genome. HIV-1 DNA and RNA were detected prior to administration of immunoglobulin in all patients. No viral nucleic acid could be detected in serum after the first transfusion in any patient and this remained so for the length of the study in ARC patients. Viral sequences were detected in 3/7 AIDS patients towards the end of the trial (10-11 months). HIV antigen was not detected, except in 2 AIDS patients towards the end of the trial. These results indicate that hyperimmune plasma eliminated detection of HIV nucleic acid from the plasma of both AIDS and ARC patients although the effect was not as sustained in AIDS. Passive immunization may be useful in clearing plasma viremia, particularly in ARC patients. In a prospective study, serum and cells from 50 patients enrolled in a controlled study for evaluation of the therapeutic value of hyperimmune plasma, are being analyzed by PCR for HIV DNA and RNA.