VIRAL DETERMINANTS OF HIV-1 PATHOGENESIS BY CONSTRUCTION OF RECOMBINANT VIRUSES

通过构建重组病毒确定 HIV-1 发病机制的病毒决定因素

• 批准号: 3804854
• 负责人: I K HEWLETT
• 金额: --
• 依托单位: CENTER BIOLOGICS EVALUATION RESEARCH TRANSFUSION
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3804854
• 关键词: human immunodeficiency virus 1; human tissue; pathologic process; transfection; virulence; virus replication

Although HIV-1 and HIV-2 share 40-60% homology at the nucleotide level, the pathogenesis of the viral infections are fairly divergent. Again, HTLV-II and HIV-1 are both T cell tropic viruses that replicate very differently in their target cells, the former causing the host to become transformed and the latter having a marked cytopathic effect. Specific viral determinants may govern the different pathogenicities of these viruses in their host cells. We have initiated studies involving the construction of recombinant viruses that have a single gene replacement of a given virus type. Target sequences that are currently being investigated are in the LTR, tat, rev, env, and integrase genes. These recombinants viruses will be transfected into the host cell and the effects of these substitutions on viral replication will be studied.

虽然HIV-1和HIV-2在核苷酸水平上有40-60%的同源性， 病毒感染的发病机制是相当不同的。 同样，HTLV-II 和HIV-1都是嗜T细胞的病毒，它们的复制方式非常不同， 它们的靶细胞，前者导致宿主转化， 后者具有显著的细胞病变效应。 特异性病毒决定簇 可能决定了这些病毒在其宿主中的不同致病性 细胞 我们已经启动了涉及构建重组 具有给定病毒类型的单个基因替换的病毒。 目标 目前正在研究的序列在LTR，达特，rev， env和整合酶基因。 这些重组病毒将被转染 以及这些取代对病毒的影响。 将研究复制。