DETECTION OF MULTIPLE VIRUSES BY PCR

PCR 检测多种病毒

• 批准号: 3792438
• 负责人: I K HEWLETT
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3792438
• 关键词: AIDS; AIDS /HIV test; HIV infections; communicable disease diagnosis; diagnosis design /evaluation; hepatitis B; hepatitis B virus group; hepatitis C virus; human immunodeficiency virus 1; human immunodeficiency virus 2; natural gene amplification; nucleic acid probes; nucleic acid repetitive sequence; polymerase chain reaction; virus classification

We have used PCR simultaneously detect multiple viruses in a single PCR assay. Briefly, co-amplification was performed with primers derived from the gag region of HIV-1 (SK 38/39) and the LTR region of HIV-2 (SK 89/90), HIV-1 and HCV (hepatitis C virus) and reaction. Amplification was performed with primer pairs specific for a set of 2 of these viruses (HIV- 1/HIV-2, HIV-1/HCV, HIV-1/HBV) and the products analyzed by hybridization with virus-specific probes. Fewer than 10 copies of each viral DNA could be detected by this method. This assay was used to detect the respective viruses in co-infected clinical samples. Abstracts describing this work will be presented at the International Congress of Virology, Berlin, Germany and the meeting on Liver Diseases. A manuscript has been submitted to the Journal of Clinical Microbiology and another manuscript is in preparation.

我们已经使用PCR在一个PCR中同时检测多种病毒 比色法 简而言之，共扩增用来源于以下的引物进行： HIV-1的gag区 (SK 38/39）和HIV-2的LTR区（SK 89/90）， HIV-1和HCV（丙型肝炎病毒）和反应。 扩增 用特异于一组2种这些病毒（HIV-1）的引物对进行检测。 1/HIV-2、HIV-1/HCV、HIV-1/HBV）和通过杂交分析的产物 病毒特异性探针 每种病毒的DNA只有不到10个拷贝 用这种方法检测。 该测定用于检测相应的 共感染临床样本中的病毒。 描述这项工作的摘要将在国际 德国柏林病毒学大会和肝病会议。 一 手稿已提交给临床微生物学杂志， 另一份手稿正在编写中。