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DETECTION OF MULTIPLE VIRUSES BY PCR

DETECTION OF MULTIPLE VIRUSES BY PCR
PCR 检测多种病毒
批准号：
3792438
负责人：
I K HEWLETT
金额：
$0.0万
依托单位：
DIVISION OF BASIC SCIENCES - NCI
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3792438
关键词：
AIDS AIDS /HIV test HIV infections communicable disease diagnosis diagnosis design /evaluation hepatitis B hepatitis B virus group hepatitis C virus human immunodeficiency virus 1 human immunodeficiency virus 2 natural gene amplification nucleic acid probes nucleic acid repetitive sequence polymerase chain reaction virus classification

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英文摘要
We have used PCR simultaneously detect multiple viruses in a single PCR assay. Briefly, co-amplification was performed with primers derived from the gag region of HIV-1 (SK 38/39) and the LTR region of HIV-2 (SK 89/90), HIV-1 and HCV (hepatitis C virus) and reaction. Amplification was performed with primer pairs specific for a set of 2 of these viruses (HIV- 1/HIV-2, HIV-1/HCV, HIV-1/HBV) and the products analyzed by hybridization with virus-specific probes. Fewer than 10 copies of each viral DNA could be detected by this method. This assay was used to detect the respective viruses in co-infected clinical samples. Abstracts describing this work will be presented at the International Congress of Virology, Berlin, Germany and the meeting on Liver Diseases. A manuscript has been submitted to the Journal of Clinical Microbiology and another manuscript is in preparation.
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