DNA ANALYSIS OF PARASITES

寄生虫的 DNA 分析

• 批准号: 3768777
• 负责人: T E NASH
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3768777
• 关键词: DNA; Giardia; antibody; chimeric proteins; human tissue; method development; microorganism classification; polymerase chain reaction; protein structure; protozoal antigen

A PCR technique employing relatively conserved regions of variant- specific surface antigen(VSP) genes was developed to differentiate Giardia isolates. The regions of the VSPH7 protein responsible for Zn and mental binding was determined by the use of fusion peptides containing various regions of VSPH7. Although the previously defined LIM- RING finger-like region bound Zn, other regions also could bind metals, and this was most likely due to CXXCXnCXXXC motifs scattered throughout the protein. There is conservation of spacing between CXXC motifs in a subset of VSPs. These findings indicate a conservation of structure, but diversity in antigenicity. Prior studies identified a transcript (GLORF- C4) specific for Group 3 Giardia; however, antibodies produced to the fusion protein identified a similar protein in other groups. Therefore, homologous proteins are present in other Giardia groups, and the reason the transcripts are not identified is because the sequences differ. Other proteins have also be identified which differ among Giardia lamblia isolates.

采用相对保守的变异区的PCR技术， 特异性表面抗原（VSP）基因被开发用于区分 贾第虫分离株。 VSPH 7蛋白负责Zn的区域 并通过使用融合肽测定金属结合 包含VSPH 7的不同区域。虽然先前定义的LIM- RING指状区域结合Zn，其它区域也能结合金属， 这很可能是由于散布在各处的CXXCXnCXXXC基序造成的 蛋白质。CXXC基序之间的间距在一个 VSP的子集。这些发现表明了结构的保守性，但 抗原性的多样性。先前的研究确定了一个转录本（GLORF- C4）特异性组3贾第虫;然而，抗体产生的 融合蛋白在其他组中鉴定出类似的蛋白。因此，我们认为， 同源蛋白质存在于其他贾第虫群中， 转录本没有被鉴定是因为序列不同。 还鉴定了其它在蓝氏贾第鞭毛虫中不同的蛋白质 分离株