IMMUNOCHEMISTRY OF PARASITIC DISEASES

寄生虫病的免疫化学

• 批准号: 3960457
• 负责人: T E NASH
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3960457
• 关键词: Cryptosporidium; Giardia; antibody; antigen antibody reaction; enzyme linked immunosorbent assay; filariasis; giardiasis; helminthic antigen; human subject; human tissue; humoral immunity; immunochemistry; immunofluorescence technique; immunoglobulin G; immunoglobulin M; microorganism culture; parasitic disease diagnosis; parasitic diseases; protozoal antigen; radioimmunoassay; schistosomiasis; serology /serodiagnosis; surface antigens; ultrasonography

The pathogenicity of Giardia lamblia for man was definitively established. 10/10 individuals inoculated with GS/E became infected and 50% became ill. Differences in the pathogenicity of isolates was additionally demonstrated because none of the 5 Isr inoculated volunteers became infected. IgM responses appear to be the most reliable humoral indicator of infection since all infected persons showed significant IgM antibody responses. Giardia antigens were reliably detected in the stools of infected volunteers despite the variation in cyst excretion. Analysis of the surface antigens and E-S products of new Giardia isolates confirms the diverse heterogeniety of Giandia lamblia in nature. The ability of Giardia to persist in culture despite the presence of high concentrations of antibiotics was studied. Persistance was dependent on the concentration of antibotics, duration of exposure, inoculum size and stability of the antibiotic. Ultrasonography reliably detects Symmers' fibrosis. This technique is now being applied in the study of infected populations in the field. An assay to detect circulating GASP antigen in humans was established. Antibody responses to specific Cryptosporidium antigens was studied using Western blots. Most infected persons recognized a 23kd antigen.

蓝氏贾第鞭毛虫对人类的致病性已明确确立。 10/10接种GS/E的个体被感染，50%患病。 此外，还证明了分离株致病性的差异 因为5名接种ISR的志愿者中没有一人被感染。 IgM 反应似乎是感染的最可靠的体液指标 因为所有感染者都表现出明显的IgM抗体反应。 在感染者的粪便中可靠地检测到贾第虫抗原， 志愿者，尽管囊肿排泄的变化。 分析 新贾第虫分离株的表面抗原和E-S产物证实了 蓝氏贾第鞭毛虫在自然界中的多样性。 贾第虫的能力 尽管存在高浓度的 抗生素进行了研究。 持久性取决于 抗生素，暴露时间，接种量和稳定性 抗生素 超声检查能可靠地检测出Symmers纤维化。 这项技术现在 被应用于现场感染人群的研究。 测定 建立了检测人体循环GASP抗原的方法。 使用免疫荧光法研究了特异性隐孢子虫抗原的抗体应答。 Western印迹。 大多数感染者识别23 kd抗原。