IMMUNOPATHOLOGY IN EYES WITH EXPERIMENTAL AND CLINICAL OCULAR DISEASES
IMMUNOPATHOLOGY IN EYES WITH EXPERIMENTAL AND CLINICAL OCULAR DISEASES
批准号:
2574498
负责人:
C-C CHAN
金额:
$0.0万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
关键词:
AIDS B lymphocyte autoimmune disorder clinical research cytokine disease /disorder model eye disorder eye disorder diagnosis human tissue immunocytochemistry immunopathology in situ hybridization inflammation laboratory mouse leukocyte activation /transformation lymphoma nitric oxide retina disorder transforming growth factors uveitis
中文摘要
免疫活性细胞的身份和拓扑定位,
动物眼常驻细胞标志物的变化
用免疫组化方法分析各种实验性葡萄膜炎
和原位杂交。 在此之前,我们已经证明了T
淋巴细胞,特别是CD4细胞,是主要的
实验性自身免疫性葡萄膜视网膜炎(EAU)中的浸润细胞,
实验性黑色素蛋白诱导的葡萄膜炎(EMIU),但这两个巨噬细胞
中性粒细胞是主要的浸润细胞
在内毒素诱导的葡萄膜炎(EIU)和所有类型的白细胞,
存在于小鼠实验性睑缘炎中。 细胞因子(例如,
淋巴因子)和炎症介质(例如,一氧化氮)发挥作用,
在葡萄膜炎的免疫发病机制中起重要作用。 表达
主要组织相容性II类抗原和粘附分子
在炎症过程中在眼驻留细胞上观察到。
我们研究了转化生长因子-β(TGF-β 1),
调节啮齿动物EMIU、小鼠EIU和
TGF-β 1转基因小鼠。 TGF-β 1通过以下途径抑制EMIU复发
Th1细胞因子向Th2细胞因子的转变。 全身性TGF-β 1抑制EIU
和血清IL-6水平。 进行性白内障和一过性视网膜水肿
在TGF-β 1转基因小鼠中发育。 无显著
在EIU的诱导中,转基因组和非转基因组之间存在差异。
小鼠和野生型对照。 我们得出结论,TGF-β 1
通过复杂的细胞因子网络调节炎症,
用于治疗人葡萄膜炎。 其他细胞因子和
炎性介质也可能有益于治疗。 一氧化氮
保护眼睛免受弓形虫感染并限制免疫反应。
IL-12抑制EIU。
来自患有各种疾病的人眼组织的标本,包括
葡萄膜炎、视网膜和角膜疾病、肿瘤和代谢遗传
疾病,通过使用常规组织学,
免疫组织化学和原位杂交技术。 在葡萄膜炎中,
免疫活性细胞和细胞因子
(e.g.,感染性微生物),是进行临床
诊断和了解疾病的发病机制。 高程
白细胞介素-10在玻璃体中的表达有助于诊断眼内大B细胞
淋巴瘤,经常伪装成特发性葡萄膜炎。 我们有
报道了转移性脉络膜细菌性脓肿发生在艾滋病,
痤疮丙酸杆菌细胞壁增厚可能与
宿主对细菌杀灭和降解的抗性
中性粒细胞和巨噬细胞。 阐明免疫病理学作用
浸润细胞与眼驻留细胞的关系
将有助于我们更好地了解和管理各种眼
患者的疾病。
英文摘要
The identity and topographic localization of immunocompetent cells and
the alteration of cellular markers on ocular resident cells in animals
with various experimental uveitis were analyzed by immunohistochemistry
and in situ hybridization. Previously, we have demonstrated that T
lymphocytes, in particular the CD4 cells, were the predominant
infiltrating cells in experimental autoimmune uveoretinitis (EAU) and
experimental melanin-protein induced uveitis (EMIU), yet both macrophages
and polymorphonuclear neutrophils were the predominant infiltrating cells
in endotoxin-induced uveitis (EIU) and all types of leukocytes were
present in murine experimental blepharitis. Cytokines (e.g.,
lymphokines) and inflammatory mediators (e.g., nitric oxide) play an
important role in the immunopathogenesis of uveitis. Expressions of
major histocompatibility class (MHC) II antigens and adhesion molecules
are observed on ocular resident cells during the inflammatory process.
We have studied transforming growth factor-beta (TGF-beta1), one of the
cytokines that modulates inflammation in rodent EMIU, murine EIU, and
TGF-beta1 transgenic mice. TGF-beta1 inhibits the recurrence of EMIU by
shifting Th1 to Th2 cytokine profile. Systemic TGF-beta1 suppresses EIU
and serum IL-6 levels. Progressive cataract and transient retinal edema
develop in TGF-beta1 transgenic mice. However, no significant
differences in the induction of EIU were found between the transgenic
mice and the wild-type controls. We have concluded that TGF-beta1
regulates inflammation through the complex cytokine network and may be
useful for the treatment of human uveitis. Other cytokines and
inflammatory mediators may also be beneficial for therapy. Nitric oxide
protects against ocular Toxoplasma gondii and limits the immune response.
IL-12 inhibits EIU.
Specimens from human ocular tissues with various diseases, including
uveitis, retinal and corneal diseases, tumors, and metabolic genetic
disorders, are studied by using routine histological,
immunohistochemical, and in situ hybridization techniques. In uveitis,
immunocompetent cells and cytokines, in addition to the stimulators
(e.g., infectious organisms), are valuable adjuncts for making a clinical
diagnosis and understanding the pathogenesis of the diseases. Elevation
of IL-10 in the vitreous helps to diagnose intraocular large B-cell
lymphoma, which frequently masquerades as idiopathic uveitis. We have
reported that metastatic choroidal bacterial abscess occurs in AIDS, and
the thickened cell wall structure of Propionibacterium acnes may relate
to the resistance of bacterial killing and degradation by the host
neutrophils and macrophages. Elucidating the immunopathological role of
the relationship between the infiltrating cells and ocular resident cells
will help us gain a better understanding and management of various ocular
diseases in patients.
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会议论文
IMMUNOPATHOLOGY IN THE EYES WITH EXPERIMENTAL AUTOIMMUNE UVEITIS
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批准号:3941666
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C-C CHAN
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依托单位:
IMMUNOPATHOLOGY IN EYES WITH EXPERIMENTAL AND CLINICAL OCULAR DISEASES
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批准号:3755558
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C-C CHAN
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依托单位:
POST-INFLAMMATORY COMPLICATIONS IN UVEITIS
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批准号:3918824
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C-C CHAN
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依托单位:
IMMUNOPATHOLOGY OF OCULAR ONCHOCERCIASIS AND OTHER PARASITIC DISEASE
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批准号:3941668
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C-C CHAN
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依托单位:
CYTOKINES AND OCULAR ANTIGENS IN THE EYE
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批准号:3877080
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C-C CHAN
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依托单位:
IMMUNOPATHOLOGY IN THE EYES WITH EXPERIMENTAL UVEITIS
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批准号:3856041
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C-C CHAN
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依托单位:
SYMPATHETIC OPHTHALMIA--IMMUNOPATHOLOGICAL FINDINGS
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批准号:3898143
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C-C CHAN
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依托单位:
POST-INFLAMMATORY COMPLICATIONS IN UVEITIS
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批准号:3898144
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C-C CHAN
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依托单位:
IMMUNOPATHOLOGY OF OCULAR ONCHOCERCIASIS
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批准号:3965397
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C-C CHAN
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依托单位:
EXPRESSION OF CLASS II ANTIGENS IN OCULAR INFLAMMATORY DISORDERS
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批准号:3965394
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C-C CHAN
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依托单位:
CYTOKINES AND OCULAR ANTIGENS IN THE EYE
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批准号:3841239
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C-C CHAN
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依托单位:
IMMUNOPATHOLOGY OF OCULAR DISEASES IN HUMANS
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批准号:3877061
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C-C CHAN
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依托单位:
IMMUNOPATHOLOGY IN THE EYES WITH EXPERIMENTAL AUTOIMMUNE UVEITIS
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批准号:3918822
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C-C CHAN
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依托单位:
SYMPATHETIC OPHTHALMIA--IMMUNOPATHOLOGICAL FINDINGS
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批准号:3918823
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C-C CHAN
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依托单位:
IMMUNOPATHOLOGY OF OCULAR DISEASES
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批准号:3941679
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C-C CHAN
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依托单位:
KINETICS OF T-LYMPHOCYTES IN THE EYES WITH EXPERIMENTAL AUTOIMMUNE UVEITIS
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批准号:3965393
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C-C CHAN
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依托单位:
POST-INFLAMMATORY COMPLICATIONS IN UVEITIS
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批准号:3965396
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:C-C CHAN
-
依托单位:
SYMPATHETIC OPHTHALMIA--IMMUNOPATHOLOGICAL FINDINGS
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批准号:3965395
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:C-C CHAN
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依托单位:
SYMPATHETIC OPHTHALMIA--IMMUNOPATHOLOGICAL FINDINGS
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批准号:3941667
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C-C CHAN
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依托单位:
IMMUNOPATHOLOGY IN THE EYES WITH EXPERIMENTAL UVEITIS
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批准号:3777628
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:C-C CHAN
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依托单位:
海外基金