STUDIES OF CENTRAL NERVOUS SYSTEM FUNCTIONAL ANATOMY

中枢神经系统功能解剖学研究

• 批准号: 3944678
• 负责人: M HERKENHAM
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3944678
• 关键词: anesthesia; autoradiography; brain mapping; brain metabolism; central nervous system; deoxyglucose; evolution; histochemistry /cytochemistry; immunochemistry; naloxone; neuroanatomy; neuropharmacology; opiate alkaloid; optics; phencyclidine; postmortem; psychobiology; radiotracer; sex behavior; synapses; tissue /cell culture; tissue /cell preparation; tritium

A sensitive method for light microscopic localization of brain receptors by in vitro autoradiography was developed previously in this laboratory. By this method we have mapped the locations of drug and neurotransmitter receptors in the brains of rats and other vertebrates, including primates. Immunohistochemistry is used to compare the locations of transmitter-specific pathways with the locations of the relevant receptors. The non- correspondence, or "mismatch" between the locations of receptors and transmitters in the opiate and tachykinin systems, as well as others, allows hypotheses about parasynaptic circuitry in the central nervous system. Physiological activation of vasopressin dynorphin pathways in the posterior pituitary altered kappa opiate receptor binding in the neurohypophysis, a site of parasynaptic communication. Current and planned work uses similar strategies to better understand the forms of functional intercellular communication.

一种灵敏的脑组织光镜定位方法 受体的体外放射自显影是以前在 这个实验室。 通过这种方法，我们绘制了 大鼠大脑中的药物和神经递质受体， 其他脊椎动物，包括灵长类。 免疫组织化学 用于比较特定传导通路的位置 相关受体的位置。 非- 受体位置之间的对应或“错配” 以及阿片和速激肽系统中的递质， 其他人，允许关于副突触回路的假设， 中枢神经系统 加压素的生理激活 垂体后叶强啡肽通路改变κ阿片 受体结合的神经垂体，一个网站的副突触 通信 目前和计划中的工作采用类似的战略 为了更好地理解功能性细胞间 通信