STUDIES OF CENTRAL NERVOUS SYSTEM FUNCTIONAL ANATOMY

中枢神经系统功能解剖学研究

• 批准号: 3968484
• 负责人: M HERKENHAM
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3968484
• 关键词: anesthesia; autoradiography; brain mapping; brain metabolism; central nervous system; deoxyglucose; evolution; histochemistry /cytochemistry; immunochemistry; naloxone; neuroanatomy; neuropharmacology; opiate alkaloid; optics; phencyclidine; postmortem; psychobiology; radiotracer; sex behavior; synapses; tissue /cell culture; tissue /cell preparation

A sensitive method for light microscopic localization of brain receptors by in vitro autoradiography was developed previously in this laboratory. By this method we have mapped the locations of drug and neurotransmitter receptors in the brains of rats and other vertebrates, including primates. Radiolabeled ligand binding using subtype-selective assay conditions confirmed the existence of anatomically distinct mu, delta, and kappa opiate receptor subtypes. A similar strategy led to the visualization of separate tachykinin receptors marked by radiolabeled substance P and eledoisin. Immunohistochemistry is used to compare the distributions of putative neurotransmitters and their receptors. The relationship of these localization patterns with other markers of brain heterogeneity, provided by tract tracing and enzyme staining, allows hypotheses about functional circuitry in the central nervous system. Physiological activation of neurochemically defined systems may lead to receptor occupation or regulation, which can be revealed and localized by in vivo as well as in vitro autoradiographic techniques.

一种灵敏的脑受体光镜定位方法 体外放射自显影术是本实验室以前开发的。 通过 这种方法我们已经绘制了药物和神经递质的位置， 受体在大鼠和其他脊椎动物的大脑中，包括灵长类动物。 使用亚型选择性测定条件的放射性标记配体结合 证实了解剖学上不同的mu，delta和kappa的存在 阿片受体亚型 一个类似的战略导致了可视化的 由放射性标记的P物质标记的单独速激肽受体， 艾杜辛。 免疫组化用于比较 假定的神经递质及其受体。 这些关系 定位模式与其他大脑异质性标志物，提供 通过道跟踪和酶染色，允许关于功能的假设， 中枢神经系统的回路 生理激活 神经化学定义的系统可能导致受体占据或 调节，这可以揭示和本地化，在体内以及在 体外放射自显影技术。