STUDIES OF CENTRAL NERVOUS SYSTEM FUNCTIONAL ANATOMY

中枢神经系统功能解剖学研究

• 批准号: 4696389
• 负责人: M HERKENHAM
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4696389
• 关键词: anesthesia; autoradiography; brain mapping; brain metabolism; central nervous system; circadian rhythms; deoxyglucose; evolution; histochemistry /cytochemistry; human tissue; immunochemistry; naloxone; neuroanatomy; neuropharmacology; opiate alkaloid; optics; phencyclidine; postmortem; psychophysiology; radiotracer; sex behavior; sleep; synapses; tissue /cell preparation

A sensitive method for light microscopic localization of brain receptors by in vitro autoradiography was developed previously in this laboratory. By this method we have mapped the locations of opiate receptors in the brains of rats and other vertebrates, including primates. Comparisons of radiolabeled opiate alkaloid binding with radiolabeled enkephalin binding have confirmed the existence of opiate receptor subtypes. A similar strategy led to the optimization of binding conditions to selectively reveal the distribution of substance P receptors and eledoisin binding sites. Immunohistochemistry is used to compare the distributions of putative neurotransmitters and their receptors. The relationship of these localization patterns with other markers of brain heterogeneity, provided by tract tracing and enzyme staining, allows hypotheses about functional circuitry in the central nervous system.

一种灵敏的脑受体光镜定位方法 体外放射自显影术是本实验室以前开发的。 通过 我们用这种方法绘制了大脑中阿片受体的位置 老鼠和其他脊椎动物包括灵长类动物 比较 放射性标记阿片类生物碱与放射性标记脑啡肽结合 已经证实了阿片受体亚型的存在 类似的 策略导致结合条件的优化，以选择性地 揭示P物质受体的分布和eledoisin结合 网站. 免疫组化用于比较 假定的神经递质及其受体。 这些关系 定位模式与其他大脑异质性标志物，提供 通过道跟踪和酶染色，允许关于功能的假设， 中枢神经系统的回路