GLYCOSYL COMPOSITION ANALYSIS OF YEAST CELL WALL MATERIAL

酵母细胞壁材料的糖基组成分析

• 批准号: 6339272
• 负责人: ROBERTA K MERKLE
• 金额: $ 0.12万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6339272
• 关键词: bacteria; biomedical resource; carbohydrates; skin; spectrometry

The sample was analyzed for monosaccharide composition by preparing the trimethylsilyl derivatives of the methylglycosides followed by gas chromatography (GC) and combined gas chromatography/mass spectrometry (GC-MS) analysis. TMS methylglycosides were prepared from the sample by methanolysis in 3 M HCl followed by N-acetylation with pyridine and acetic anhydride. The samples were then treated with Tri-Sil. GC analysis of the TMS methylglycosides was done on an HP5890 GC using a DB1 fused silica capillary column. GC-MS analysis was performed using an HP5890 GC coupled to a 5970 MSD. Monosaccharides were identified by their retention times in comparison to standards, and the carbohydrate character of these was authenticated by their mass spectra.

通过以下方法分析样品的单糖组成： 制备甲基糖苷的三甲基硅基衍生物， 然后进行气相色谱（GC）和合并的气体 色谱/质谱（GC-MS）分析。 TMS 通过在3 M甲醇中甲醇分解从样品制备甲基糖苷 HCl，然后用吡啶和乙酸酐进行N-乙酰化。 的 然后用Tri-Sil处理样品。 TMS的GC分析 甲基糖苷在HP 5890 GC上使用DB 1熔融二氧化硅 毛细管柱 使用HP 5890 GC进行GC-MS分析 与5970型默沙东手枪相连 单糖通过其 与标准品相比的保留时间，以及碳水化合物 这些化合物的质谱特征得到了证实。