SEMEN COLLECTION FROM MALE BABOONS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 雄性狒狒的直肠探针电射精已被证明是可靠的，可以产生新鲜、高质量的精子样本，用于辅助生殖技术（ART）研究。为了获得狒狒精子样本用于发育生殖研究和干细胞研究，指定的雄性狒狒通过直肠探针电射精提供精液样本用于精子分离。 五个确定的狒狒精子使用领域包括： 1. 通过卵泡抽吸从经尿道刺激的雌性狒狒收集的卵母细胞的体外受精。（抽吸程序当天所需的精子;种内受精） 2. 非计划性尸检卵巢卵母细胞的体外受精。（在尸检日期，精子需要从卵母细胞分离48小时;种内和种间受精） 3. 当取卵当天无法获得猕猴或黑猩猩精子时，猕猴（恒河猴;食蟹猴）或黑猩猩卵母细胞受精研究的替代精子来源。（卵母细胞采集日所需精子;种间受精） 4. 精子在液氮中的冷冻保存和储存。 5. 用于研究调查的新鲜精子采集（即测试新抗体[免疫细胞化学];生物化学研究;等）。 根据当前机构标准操作规程的要求，要求的狒狒将在射精程序之间获得休息期，并由兽医进行监测。对于成功产生干细胞系的男性，我们可能会要求2-3份皮肤样本（约5 mm活检穿孔）、3- 5 ml全血（凝块管）和/或3- 5 ml全血（EDTA管），用于基因分型或男性与已建立细胞系之间的其他遗传比较。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Rectal probe electro-ejaculation of male baboons has proven reliable for producing fresh, high quality sperm samples for investigative use in assisted reproductive technologies (ART) studies. In order to obtain baboon sperm samples for developmental reproductive studies and stem cell research, assigned male baboons provided semen samples via rectal probe electro-ejaculation for sperm isolation. Five identified areas for the use of baboon sperm include: 1. In vitro fertilizations of oocytes collected via follicular aspirations from hormonally stimulated female baboons. (Sperm required day of aspiration procedures; intra-species fertilizations) 2. In vitro fertilizations of oocytes from unplanned necropsy ovaries. (Sperm required 48hr from oocyte isolations on necropsy date; intra & inter-species fertilizations) 3. Alternative sperm source for fertilization studies in either macaques (rhesus; cynomolgus) or chimpanzee oocytes when neither macaque nor chimpanzee sperm is unattainable on day of oocyte retrieval. (Sperm required day of oocyte collections; inter-species fertilizations) 4. Cryopreservation and banking of sperm in liquid nitrogen. 5. Fresh sperm collections for research investigations (i.e. testing new antibodies [immunocytochemistry]; biochemistry studies; etc). The requested baboons will be allowed rest periods between ejaculation procedures as mandated by the current facility standard operating procedure and monitored by a veterinarian. For males that successfully produce stem cell lines, we may request 2-3 skin samples (~5mm biopsy punch), 3-5mls of whole blood in clot tube and/or 3-5mls of whole blood in EDTA tube for genotyping or other genetic comparisons between the male and the established cell lines.