Gene Expression Profiling and Clinical Characterization of Phototoxicity

光毒性的基因表达谱和临床特征

• 批准号: 8349232
• 负责人: Heidi Kong
• 金额: $ 3.38万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8349232
• 关键词: Biopsy; Clinical; Cutaneous; Cytochrome P450; Data; Diuretics; Doxycycline; Gene Expression; Gene Expression Profiling; Genotype; Goals; Immunohistochemistry; Light; Microarray Analysis; Non-Steroidal Anti-Inflammatory Agents; Pathway interactions; Pharmaceutical Preparations; Phototoxicity; Protocols documentation; Reaction; Recommendation; Role; Signal Transduction; Skin; Sunscreening Agents; Testing; Ultraviolet Rays; Visible Radiation; Voriconazole; antimicrobial; healthy volunteer; translational approach; ultraviolet

In fiscal year 2011, we continued to validate our microarray data obtained from skin biopsies of skin phototoxicity. The initial analysis demonstrated that there were gene expression signals in skin treated with doxycycline alone (without ultraviolet or light exposure) compared to control skin. We amended our protocol in 2010 to allow us to re-treat healthy volunteers with doxycycline to obtain additional biopsies. We treated 4 additional healthy volunteers in fiscal year 2011. We have been performing immunohistochemistry on these additional biopsies to validate the microarray data.

在2011财年，我们继续验证从皮肤光毒性活检中获得的微阵列数据。初步分析表明，与对照皮肤相比，单独使用强力霉素处理（无紫外线或光照）的皮肤中存在基因表达信号。我们在2010年修改了我们的方案，允许我们用强力霉素重新治疗健康志愿者，以获得额外的活检。在2011财政年度，我们又治疗了4名健康志愿者。我们一直在对这些额外的活检进行免疫组织化学来验证微阵列数据。