Gene Expression Profiling and Clinical Characterization of Phototoxicity

光毒性的基因表达谱和临床特征

• 批准号: 10710338
• 负责人: Heidi Kong
• 金额: $ 1.76万
• 依托单位: NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10710338
• 关键词: Antibiotics; Biopsy; Clinical; Clinical Research; Cutaneous; Cytochrome P450; Diuretics; Doxycycline; Gene Expression; Gene Expression Profiling; Genotype; Goals; Human Microbiome; Ingestion; Investigation; Light; Microarray Analysis; Non-Steroidal Anti-Inflammatory Agents; Pathway interactions; Pharmaceutical Preparations; Phototoxicity; Protocols documentation; Publishing; Reaction; Recommendation; Role; Sampling; Serum; Skin; Stains; Sunscreening Agents; Testing; UV Radiation Exposure; Ultraviolet Rays; Visible Radiation; Voriconazole; antimicrobial; base; experience; healthy volunteer; microbiome alteration; response; translational approach; ultraviolet

In 2010, the protocol was amended to allow us to treat a subset of returning healthy volunteers with doxycycline to obtain additional biopsies. In 2011, we completed treatment of the additional healthy volunteers. In 2012, we completed the analysis of immunohistochemical staining of the additional biopsies obtained from healthy volunteers treated with doxycycline. In fiscal year 2013, we investigated the serum levels of doxycycline from the healthy volunteers who participated in the study in previous years. In fiscal year 2017, the protocol was amended to allow additional investigation of skin samples post-UV exposure which were inconclusive. Based upon the clinical research experience from treating healthy volunteers with doxycycline, a protocol was subsequently developed to analyze the human microbiome alterations associated with doxycycline ingestion. The findings of that study were published in fiscal year 2022.

2010年，该方案进行了修订，允许我们用强力霉素治疗一部分返回的健康志愿者，以获得额外的活检。2011年，我们完成了额外健康志愿者的治疗。2012年，我们完成了对接受强力霉素治疗的健康志愿者的额外活检组织的免疫组织化学染色分析。在2013财政年度，我们调查了前几年参与研究的健康志愿者的多西环素血清水平。在2017财年，对方案进行了修订，以允许对紫外线暴露后的皮肤样本进行额外研究，但结果不确定。基于用多西环素治疗健康志愿者的临床研究经验，随后开发了一种方案来分析与多西环素摄入相关的人类微生物组改变。该研究的结果于2022财年公布。