Studies of Skin Microflora in Healthy Individuals and Atopic Dermatitis Patients

健康人和特应性皮炎患者皮肤微生物群的研究

• 批准号: 9556405
• 负责人: Heidi Kong
• 金额: $ 41.63万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9556405
• 关键词: Adult; Archaea; Atopic Dermatitis; Bacteria; Bacteriophages; Childhood; Clinical; DNA; Dermatitis; Dermatologic; Dermatology; Disease; Eczema; Enrollment; Funding; Future; Genes; Genomic approach; Genomics; Goals; Health; Human Microbiome; Immunoglobulin Variable Region; Individual; Job' s Syndrome; Light; Medical; Metagenomics; Methods; Patients; Phylogenetic Analysis; Play; Population; Publishing; RNA; Ribosomes; Role; Sampling; Shotguns; Skin; Structure; Techniques; United States National Institutes of Health; Viral; Virus; Visit; Work; congenital immunodeficiency; experimental study; follow-up; fungus; metagenomic sequencing; microbial; microbial community; microbiome; microbiota; patient population; skin microbiome; skin microbiota

We continue to investigate and publish our findings on the microbiota in patients with atopic dermatitis in contrast to healthy individuals. We study bacterial, fungal, and viral components of the skin microbiome and have expanded our sequencing repertoire beyond bacterial amplicon sequencing to include fungal amplicon sequencing and shotgun metagenomic sequencing. We continue to study the skin microbiome in patients with atopic dermatitis and with primary immunodeficiencies associated with dermatitis (including hyper-IgE syndromes), in addition to follow-up sampling visits from previously enrolled subjects. The sequencing for this project initially was previously funded by the National Institutes of Health Roadmap Project, The Human Microbiome Project. In the last fiscal year, we have expanded our work to study these patient populations using more advanced sequencing techniques and published our findings using shotgun metagenomic sequencing of samples from atopic dermatitis patients.

我们将继续研究并发表我们对特应性皮炎患者与健康个体相比的微生物群的研究结果。我们研究皮肤微生物组的细菌、真菌和病毒组分，并将我们的测序谱扩展到细菌扩增子测序之外，包括真菌扩增子测序和鸟枪宏基因组测序。我们继续研究特应性皮炎患者和与皮炎相关的原发性免疫缺陷（包括高IgE综合征）患者的皮肤微生物组，此外还对先前招募的受试者进行了随访采样。该项目的测序最初由美国国立卫生研究院路线图项目人类微生物组项目资助。在上一个财政年度，我们扩大了我们的工作，使用更先进的测序技术研究这些患者人群，并发表了我们的研究结果，使用鸟枪宏基因组测序的样本从特应性皮炎患者。