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Fluorescence imaging upgrade for stereoscope

立体镜荧光成像升级

基本信息

批准号：
345372-2007
负责人：
Pearson, Angela
金额：
$ 2.15万
依托单位：
Institut national de la recherche scientifique
依托单位国家：
加拿大
项目类别：
Research Tools and Instruments - Category 1 (<$150,000)
财政年份：
2006
资助国家：
加拿大
起止时间：
2006-01-01 至 2007-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=326044
关键词：
Fluorescence imaging upgrade stereoscope

项目摘要

Herpes viruses infect a wide variety of animals including humans, which has a significant impact both for agriculture and human health. Herpes simplex virus is the prototypic herpesvirus. Like all herpesviruses, it is a highly complex organism that uses multiple strategies to reprogramme the infected cell to its own benefit. The goal of our research programme is to understand this process at a molecular level. One of our two main interests is to understand how viral genes are activated once a cell becomes infected, and how this infection affects which of the cell's genes are on or off. We are also studying how certain viral genes alter the normal functions of the cell. Central to our strategy for achieving each of these goals is the ability to alter the genetic material of the virus, and then to study the impact of these alterations on the ability of the virus to replicate. In order to identify such genetically modified viruses, we incorporate into their genome genes that make the infected cells fluoresce under special light. The fluorescence imaging upgrade will allow us to efficiently screen infected cells for the desired viruses. By manipulating the viral genome, we can directly test the importance of different viral genes on fundamental aspects of infections. The results of our research programme have implications for the design of better treatments for financially devastating veterinary diseases, as well as the engineering of new strains of herpes simplex virus that can be used in gene therapy strategies.

疱疹病毒感染包括人类在内的多种动物，对农业和人类健康都有重大影响。单纯疱疹病毒是典型的疱疹病毒。像所有疱疹病毒一样，它是一种高度复杂的有机体，它使用多种策略来重新编程受感染的细胞以使其受益。我们研究项目的目标是在分子水平上理解这一过程。我们的两个主要兴趣之一是了解一旦细胞被感染，病毒基因是如何被激活的，以及这种感染如何影响细胞中哪些基因是开启或关闭的。我们也在研究某些病毒基因如何改变细胞的正常功能。我们实现这些目标的核心策略是改变病毒遗传物质的能力，然后研究这些改变对病毒复制能力的影响。为了鉴定这种转基因病毒，我们在它们的基因组中加入了使受感染细胞在特殊光线下发出荧光的基因。荧光成像的升级将使我们能够有效地筛选感染细胞中所需的病毒。通过操纵病毒基因组，我们可以直接测试不同病毒基因在感染基本方面的重要性。我们的研究方案的结果对设计更好的治疗具有财政破坏性的兽医疾病的方法，以及可用于基因治疗战略的单纯疱疹病毒的新菌株的工程具有影响。