Role of the histone variant H3.3 in germ cell development

组蛋白变体 H3.3 在生殖细胞发育中的作用

• 批准号: nhmrc : 1002316
• 负责人: A/Pr Lee Wong
• 金额: $ 38.76万
• 依托单位: Murdoch Childrens Research Institute
• 依托单位国家: 澳大利亚
• 项目类别: Project Grants
• 财政年份: 2011
• 资助国家: 澳大利亚
• 起止时间: 2011-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/role-histone-variant-cell-development/108801
• 关键词: Role; histone; variant; H3.3; germ

Over their life cycle, germ cells are unique in undergoing a large scale reformatting of their gene or DNA control systems, required for their own development, and for the development of the fertilized egg. We think that the protein ïhistone H3.3Í is crucial to this reformatting process. We will test this possibility by determining how much H3.3 is present in germ cells. Also, we will make mice which lack this protein in germ cells to see if this affects the reformatting process.

在其生命周期中，生殖细胞的独特之处在于对其基因或 DNA 控制系统进行大规模重新格式化，这是其自身发育和受精卵发育所必需的。我们认为蛋白质“组蛋白 H3.3”对于这一重新格式化过程至关重要。我们将通过确定生殖细胞中存在多少 H3.3 来测试这种可能性。此外，我们将制造生殖细胞中缺乏这种蛋白质的小鼠，看看这是否会影响重新格式化过程。