Design of Bioreactors for Insect Cell Cultures for the Production of Viral Pesticides and Foreign Gene Products

昆虫细胞组织培养物可潜在地用于生产 病毒杀虫剂和生产蛋白质， 不能在细菌或酵母细胞中制造的重组DNA。 一 目前还没有实用的大规模昆虫组织培养系统 如果是的话，它可能会对美国产生巨大的经济影响。 生产上述材料。 该建议旨在制定一项 一种适合于昆虫细胞培养的新型生物反应器， 也适合规模扩大，同时提供高水平的控制 微环境。 所提出的工作是真正的创新和新颖，并符合标准 小说研究加速奖（见随附的AD/ENG公告 No. 85-25）。 PI被认为是完全有能力执行 建议的研究，我强烈建议，这一建议是 根据与提案一起提交的预算供资。

Insect cell tissue cultures can potentially be used for the production of viral insecticides and for the production of proteins obtained from recombinant DNA that can not be made in bacterial or yeast cells. A practical large scale insect tissue culture system is not yet available and if it were, it could have immense economic implications for the production of the above materials. This proposal seeks to develop a novel bioreactor suitable for the cultivation of insect cells that is also amenable to scale-up while providing a high level of control over the microenvironment. The proposed work is truly innovative and novel and meets the criteria for Expedited Awards for Novel Research (see attached AD/ENG Bulletin No. 85-25). The PIs are considered to be quite capable of carrying out the proposed research and I strongly recommend that this proposal be funded in accordance with the budget submitted with the proposal.