Acquisition of a 500 MHz NMR Spectrometer

9217413 Giedroc主要研究人员的集体研究兴趣涉及多肽和蛋白质结构以及分子识别的研究。与核酸相互作用的两种基因调控的锌金属蛋白(锌指蛋白)的金属配位方式和整体结构将被确定。结合底物的构象研究和导致人胰岛素受体酪氨酸激酶结构域自动磷酸化的结构变化被建议用来研究这类酶的催化作用。核磁共振结构研究计划对有核薄片和模型夹心蛋白质进行研究，以了解支配蛋白质中有核薄片二级结构形成的基本规则。最后，提出了合成构象受限的生物活性多肽的结构研究，如阿片类拮抗剂和活性部位多肽作为病毒蛋白酶抑制剂的候选者。请求的核磁共振仪器将成为德克萨斯农工大学生物科学与技术研究所大分子设计中心的一个组成部分。由于该中心的主要目标是招收、支持和教授优秀的本科生、研究生和研究生，拟议的仪器将用于增强和扩大他们在蛋白质结构领域的教育经验。

9217413 Giedroc The collective research interests of the primary investigators concern the study of peptide and protein structure and molecular recognition. The mode of metal coordination and overall structure of two gene regulatory zinc metalloproteins (zinc-finger proteins) which interact with nucleic acids will be determined. Conformational studies of bound substrates and structural changes which result in autophosphorylation of the tyrosine kinase domain of the human insulin receptor are proposed to study catalysis by this class of enzymes. NMR structural studies of nucleated -sheets and model -sandwich proteins are planned to understand the fundamental rules that govern the formation of -sheet secondary structures in proteins. Finally, structural studies of synthetic conformationally constrained peptidomimetics of bio-active peptides, e.g., opiate antagonists and active site peptides as candidates for viral protease inhibitors, are proposed. The request NMR instrumentation will be a component of the Center for Macromolecular Design of the Institute of Biosciences and Technology at Texas A&M University. As a major goal of the Center is the recruitment, support, and teaching of outstanding undergraduate, graduate, and postgraduate students, the proposed instrumentation will be used to enhance and expand their educational experience in the area of protein structure.