Acquisition of a Flow Cytometer for Marine Biology and Biotechnology

流式细胞仪提供了快速分析单个细胞的荧光、散射和几种化学和生化参数的独特能力。本提案要求为斯克里普斯海洋学研究所（SIO）海洋生物技术和生物医学中心购买一台流式细胞仪，供不同领域的几名研究人员共同使用。该仪器将用于提供样品。这些信息将用于了解这些生物的形态和遗传多样性，它们对水柱生物光学特性的影响，以及它们对营养和其他环境胁迫的反应。在一个新的应用中，该仪器将被用来表征目前无法培养的生物产生的次生代谢物，这些生物是通过流式细胞术分选从现场样品中分离出来的。流式细胞仪还将用于实验室培养，以研究特定物种对各种环境胁迫的生理反应。本研究是开发分子探针作为特定应力指示物和参数化海洋光合作用的光学模型作为特定应力函数的努力的一部分。

9317958 Palenik The flow cytometer provides the unique capability of rapid analyzing the fluorescence, scattering, and several chemical and biochemical parameters of individual cells. This proposal requests funds to acquire a flow cytometer for the Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography (SIO) to be jointly used by several investigators working in diverse areas. The instrument will be used to provide samples. This information will be used to understand the morphological and genetic diversity of these organisms, their effects on the biooptical properties of the water column, and their responses to nutrient and other environmental stresses. In a novel application, the instrument will be used to characterize the bioactive secondary metabolites produced by currently unculturable organisms isolated from field samples by flow cytometric sorting. The flow cytometer will also be used on laboratory cultures to study physiological responses of particular species to various environmental stresses. This research is part of efforts to develop molecular probes as indicators for particular stresses and to parameterize optical models of oceanic photosynthesis as a function of particular stresses.