Luzula elegans - a holocentric species with an inverted sequence of meiotic events?

全着丝粒染色体生物的减数分裂说明，我们的观点减数分裂染色体排列和控制减数分裂的基础上观察单着丝粒染色体可能不适用于所有生物。为了证明在全着丝点的Luzula属中减数分裂事件的顺序是否是颠倒的（第一次分裂是等式的，而第二次分裂是还原的），该项目旨在研究Luzula属中减数分裂事件的顺序。使用先进的高分辨率显微镜观察线虫。L.将鉴定编码涉及减数分裂重组、交换形成和姐妹染色单体凝聚/分离的蛋白质的线虫基因，并将它们的序列信息用于产生抗体，以将这些蛋白质定位在减数分裂染色体上。具体而言，将分析负责减数分裂I-II过渡期间同源（非姐妹）染色单体的端对端缔合的染色质结构域以及减数分裂II中同源染色单体之间的比对类型。染色体特异性FISH标记将用于追踪减数分裂期间单个染色体的动态。

Meiosis in organisms with holocentric chromosomes illustrates that our views of meiotic chromosome arrangement and control of meiosis based on observations of monocentric chromosomes may not apply to all organisms. To proof whether in the holocentric genus Luzula the sequence of meiotic events is inverted (first division is equational whereas the second division is reductional), the project aims to investigate the sequence of meiotic events in L. elegans using advanced high-resolution microscopy. L. elegans genes encoding proteins involved in meiotic recombination, crossover formation and sister chromatid cohesion/separation will be identified and their sequence information will be used to generate antibodies for localisation of these proteins on meiotic chromosomes. In particular, the chromatin domain responsible for the end-to-end association of homologous (non-sister) chromatids during meiosis I - II transition and the type of alignment between homologous chromatids in meiosis II will be analysed. Chromosome-specific FISH marker will be used to trace the dynamics of individual chromosomes during meiosis.