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A simple system for developing the microsatellite markers using RSE-PCR and pyrosequencing

A simple system for developing the microsatellite markers using RSE-PCR and pyrosequencing
使用 RSE-PCR 和焦磷酸测序开发微卫星标记的简单系统
批准号:
24658138
负责人:
SHIRAISHI Susumu
金额:
$2.5万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2012
资助国家:
日本
项目状态:
已结题
起止时间:
2012 至 --

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中文摘要
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英文摘要
DNA molecular markers were widely utilized as powerful tool in breeding, genetics, and ecology. Among these markers, microsatellite marker is highly variable, and possesses a large quantity of genetic information. Constructing a new system, a next generation sequencer (pyrosequencing) was introduced to gain the huge sequences of microsatellite regions in genomes. The new system showed the possibility for a simple and inexpensive microsatellite development.
期刊论文(4)
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会议论文
DOI: 10.1007/s12686-012-9814-3
发表时间: 2012
期刊: Conservation Genetics Resources
影响因子: 1.1
作者: [D. V. Thao, M. Yamashita, A. Watanabe, S. Shiraishi]
通讯作者: S. Shiraishi
DOI: --
发表时间: 2013
期刊: Conservation Genet Resour
影响因子: --
作者: [Thao DV, Widyatmoko AYPBC, Guan L Watanabe A, Gotoh E, Shiraishi S]
通讯作者: Shiraishi S
A simple sequencing system of complete chloroplast genome using long PCR and NGS in coniferous species
  • 批准号:
    25660124
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
  • 资助金额:
    $2.5万
  • 财政年份:
    2013
  • 负责人:
    SHIRAISHI Susumu
  • 依托单位:
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  • 批准号:
    22658050
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
  • 资助金额:
    $2.29万
  • 财政年份:
    2010
  • 负责人:
    SHIRAISHI Susumu
  • 依托单位:
Global transcriptome analysis of the pine wilt disease and application to the resistance breeding
  • 批准号:
    21380100
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 资助金额:
    $11.23万
  • 财政年份:
    2009
  • 负责人:
    SHIRAISHI Susumu
  • 依托单位:
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  • 批准号:
    17208014
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
  • 资助金额:
    $29.12万
  • 财政年份:
    2005
  • 负责人:
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