Evaluation of genetic diversity of primate populations by AFLP as a genome typing technique and its application

在本研究中，我研究了扩增片段长度多态性（AFLP）作为一个全基因组的遗传标记，在灵长类种群的遗传多样性评价。虽然人类微卫星同源物可以应用于非人类灵长类动物，但受试物种基因组上的真实的遗传多样性不能通过这样选择的高度多态性基因座来量化。本研究首先分析了京都大学灵长类研究所饲养的猕猴的AFLP和微卫星DNA多态性，比较了它们之间的群体遗传参数，表明AFLP在检测群体间的遗传分化和对混合群体中相关个体进行聚类方面与微卫星标记具有可比性。其次，对恒河猴与猕猴杂交个体进行了AFLP分析。独眼巨人AFLP图谱的主坐标分析结果表明，杂种F_1的遗传情况较为复杂。利用线粒体DNA和TSPY基因序列分析了婆罗洲敏捷长臂猿（Hylobates agilis albibarbis）的系统发育位置，阐明了婆罗洲敏捷长臂猿（Hylobates agilis albibarbis）与苏门答腊敏捷长臂猿（H. agilis），而不是H.居住在婆罗洲的穆埃里。在系统发育分析的过程中，我发现了两种mtDNA类型（“agilis 1”和“agilis 2”）。苏门答腊产的阿吉利斯。Agilis 2型与H.拉。AFLP分析表明，H. 'agilis 2'的agilis不是H. agilis和H. agilis 1和agilis 2群体间无遗传分化，表明类lar-like mtDNA（agilis 2）已渗入Summandra H的一部分。过去的敏捷。

In this study, I examined the amplified fragment length polymorphism (AFLP) as a genome wide genetic marker in evaluation of genetic diversity in primate populations. Although human microsatellite homologues can be applied to non-human primates, the real genetic diversity over the genome of the subject species could not be quantified by the such selected highly polymorphic loci. In this study, firstly I analyzed AFLP and microsatellite DNA polymorphism in Macaca fusucata kept in Primate Research Institute, Kyoto University, and compared population genetic parameters between them to characterize AFLP as the markers, showing that AFLP was comparable to microsatellite markers in detection of genetic differentiation between populations and in clustering the related individuals from a mixed population. Secondary, I analyzed AFLP in hybrid indivisuals between Macaca fuscata and M. cyclopis. The result of principal coordinate analysis of AFLP profiles suggested more complex genetic situation in the F1 hybrid individuals. I analyzed the phylogenetic position of Bornean agile gibbons (Hylobates agilis albibarbis) using mtDNA and TSPY gene sequences, and clarified that it was a sister to Sumatran agile gibbons (H. agilis), not to H. muelleri which inhabit Borneo. In the process of this phylogenetic analysis, I found two mtDNA types ('agilis 1' and 'agilis 2') in H. agilis from Sumatra. The 'agilis 2' type was closely related to the mtDNA of H. lar. AFLP analysis elucidated that H. agilis of 'agilis 2' was not hybrid individuals between H. agilis and H. lar, and that no genetic differentiation existed between the groups of 'agilis 1 ' and 'agilis 2', suggesting the introgression of the lar-like mtDNA ('agilis 2') into a part of Sumatran H. agilis in the past.