Large-scale DNA sequencing and its application

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We established a large-scale (Sanger) DNA sequencing system through the review, improvement and cost reduction of the sequencing process step by step, leading to allow us to obtain 6 million (Sanger) reads per year. Based on the requests from the researchers in the grant group, we produced 3.4 million EST reads for 92 cDNA libraries from 16 organisms. We also produced 19.4 million reads for whole genome shotgun sequencing of 7 organisms including wild-derived mouse, a hemichordata, water bears, the choanoflagellate Monosiga ovata. Altogether 24 million reads were produced during the project period. To analyze the reads more closely, we developed a work-bench for genome finishing and a viewer for polymorphism identification. For the next generation DNA sequencing system, we set up a computer system with a security system that is essential for personal genomics and performed various DNA sequencing. Furthermore, we developed a viewer system for polymorphism identification and a system for cDNA full sequencing. Solexa multiplexing data analysis was also tested.