Studies on Genetic Resources of the silkworm, Bombyx mori

发现了新的突变体--胭脂和不蜕皮。连锁分析表明，ro基因位于性染色体上。对凝乳酶抑制因子13(CI-13)的核苷酸序列进行了测定，发现具有KI基因纯合子的成虫产下的肾形卵不能发育成完整胚胎。这些结果表明KI卵子的母体mRNAs不同于正常卵子的母体mRNAs。利用从正常和Ki突变的未受精卵中提取的mRNAs，用RT-PCR和差异显示的方法分析了它们之间的差异。结果表明，K1突变株的母本基因与正常株无明显差异，隐性突变株SoL基因与位于第6连锁群末端的E基因连锁。E^&lt；ca&gt；与F基因位于同一连锁群的三点检验表明，3个基因座的排列顺序为Sol-E^&lt；ca&gt；-F，其中Sol基因的位置为第6连锁群的-21.1 cM。为了阐明这一结果，OEDER与家蚕触角基因的RNA探针进行了原位杂交，荧光信号在染色体的内部区域检测到，而不是在其远端检测到。

New mutants, rouge and non-molting were found. The ro gene was located on the sex chromosome by linkage analysis. Nucleotide sequence of chymotrypsin inhibitor 13 (CI-13) was determined.The adult having homozygous ki gene lays kidney-shaped eggs which can not develop complete embryo. These results suggest that maternal mRNAs of the ki egg differ from maternal mRNAs of the normal egg. Using the mRNAs extracted from unfertilized eggs of a normal and ki mutant, RT-PCR and differential display methods was performed to elucidate the difference between them. The results indicated that maternal mRNA from ki mutant are not different from that of a normal..The sol gene for a recessive mutant named "soft and limp" was shown to be linked with E gene mapped to the end of the 6th linkage group. A three-point test involving the E^<ca> and the F gene located on the same linkage group indicated that the arrangement of three gene loci is in the order of sol-E^<ca>-F The locus of the sol gene was determined to be -21.1 cM of the 6th linkage group. In oeder to clarify this result, in situ hybridization with a RNA probe derived from the Bombyx mori Antennapedia gene was carried out, the fluorescence signal was detected at an internal region of a chromosome, not at its distal end.