Prevention for allergic diseases by clarification of interaction between environmental chemicals and genes of eosinophil-related protein

为了通过阐明环境与基因之间的关系来提出针对性的过敏性疾病预防方法，我们比较了嗜酸性粒细胞相关蛋白基因的单核苷酸多态性（SNP），例如，CC趋化因子受体（CCR）家族、Eotaxin/CCL 11、Eotaxin-2/CCL 24、Eotaxin-3/CCL 26、单核细胞趋化蛋白-1（MCP-1、CCL 2）在哮喘或日本雪松花粉症患者与对照之间的差异。与对照组相比，我们发现哮喘或日本雪松花粉症患者CCR 3基因51 C，CCL 26基因2497 G和2563 C以及MCP-1基因2518 G的频率显著较高。这些蛋白质的血清水平与过敏性疾病的严重程度有显着相关性。这些结果提示这些嗜酸性粒细胞相关蛋白在变态反应性疾病中的临床意义。接下来，我们开发了新的过敏预防方法，使用含有活性炭和无定形氧化铁的过滤系统，有效地去除柴油机排气颗粒物（DEP）。我们证明，去除DEP的过滤系统产生显着抑制过敏反应，如。哮喘小鼠肺组织中嗜酸性粒细胞和中性粒细胞浸润、肺部炎症和Der f特异性IgG 1抗体水平升高。因此，这种过滤系统可能是最有效的预防过敏的发展，如果它的使用开始在我们的生活中尽可能早（例如在我们的婴儿期）。

To propose tailor-made preventive methods for allergic diseases by clarification between environment and genes, we compared single nucleotide polymorphisms (SNP) of genes of eosinophil-related protein, e.g., CC chemokine receptor (CCR) family, Eotaxin/CCL11, Eotaxin-2/CCL24, Eotaxin-3/CCL26, Monocyte chemoattractant protein -1 (MCP-1, CCL2) between patients with asthma or Japanese cedar pollinosis and controls. We recognized significantly higher frequencies of 51 C in CCR3 gene, 2497G and 2563C in CCL26 gene, and 2518G in MCP-1 gene in patients with asthma or Japanese cedar pollinosis as compared to controls. There were significant correlations between serum levels of those proteins and severity of those allergic diseases. These results suggest the clinical significance of those eosinophils-related proteins in allergic diseases. Next, we developed the novel preventive methods for allergy, using the filter system incorporating activated carbon and amorphous iron(hydr)oxide, which efficiently removes diesel exhaust particulate (DEP). We demonstrated that removal of DEP by the filter system produced significant inhibition of allergic responses, e. g, eosinophils and neutrophils infiltration into lung tissues, lung inflammation and elevated Der f specific IgGl antibody level in asthma model mice BALB/c, which were sensitized and stimulated with Der f. Therefore, this filter system might be the most effective to prevent allergy development, if its use were started in our life as early as possible (e.g. in our infancy).