FIBER OPTIC LASER DOPPLER SYSTEM FOR CILIARY MOTILITY

肺细胞无创监测传感器及探针的研制 在体内的功能将提供新的信息的病理生理学 肺部疾病。 这将为预防性或 治疗性干预方法将根据 测量细胞异常。 在该I期提案中，体内， 非破坏性光纤激光多普勒系统（FOLDS）将 构建用于实时测量纤毛活动的两个参数 即纤毛搏动频率（CBF）和异时波周期（MWP）。 这些参数是有效的粘膜纤毛运输的基础。的 传感探头将穿过光纤的采样通道 支气管内窥镜 光纤原型将开发利用 动态激光光散射时频分析 和时间尺度分析（小波）。 CBF和MWP将在 天然纤毛上皮细胞和狗。 在第二阶段，该系统将 进行包装并在人体上进行测试。 我们设想这将被用于 在常规气管支气管镜检查和内窥镜鼻窦上颌 检查病人在门诊部和医院。 它 将提供第一个体内定量信息。 这将 支持定性视觉临床评估， 用于体外测定CBF的活组织检查。 建议的商业应用 该系统将用于鼻和肺部疾病， 粘膜纤毛运输受损。 该系统也适用于 评估大多数类型的生物动力学，速度流剖面的 流体以及物理振动的远程测量。

Development of non-invasive sensory and probes to monitor lung cell function in vivo will provide novel information on the pathophysiology of lung diseases. This will enable rationales for prophylactic or therapeutic intervention approaches to be developed based on the measured cellular abnormality. In this Phase I proposal, an in vivo, non-disruptive, Fiber Optics Laser Doppler system (FOLDS) will be constructed to measure two parameters of ciliary activity in realtime namely, ciliary beat frequency (CBF) and metachronal wave period (MWP). These parameters are fundamental to effective mucociliary transport. The sensing probe will pass through the sampling channel of a fiber-optic broncho-endoscope. A fiberoptic prototype will be developed utilizing dynamic laser light scattering in conjunction with the time-frequency and time-scale analyses (wavelet). CBF and MWP will be determined in native ciliated epithelia and in dogs. In Phase II, this system will be packaged and tested in humans. We envision that this will be used during routine trachael-bronchoscopy and endoscopic paranasal maxillary examination in patients in ambulatory care units and in hospitals. It will provide the first in vivo quantitative information. This will support qualitative visual clinical assessment and eliminate the need for biopsies for in vitro determination of CBF. PROPOSED COMMERCIAL APPLICATION This system will be used in nasal and lung diseases that exhibit impairment of mucociliary transport. The system is also applicable to assess most types of biological motilities, velocity flow profile of fluids as well as remote measurements of physical vibrations.