NOVEL POLYMER-COATED ION-EXCHANGE CHROMATOGRAPHY SUPPORT

本提案描述了使用射频(RF)等离子体诱导 聚合法制备新型离子交换载体 生物聚合物的高效液相色谱分离。 二氧化硅和氧化铝载体将均匀地涂覆一层薄层 含有可电离基团的可聚合单体。这个 然后将分散的单体涂层聚合到表面上 整个支撑体的孔结构形成薄的、无销孔的 具有高表面密度的可电离基团的层析载体。 已经选择了单体来同时制备弱的和强的 阳离子和阴离子交换材料的色谱学评价 将使用蛋白质和其他生物分子来表征 离子交换介质的性能。 这项第一阶段工作的成功完成将导致 高选择性聚合物涂层分离介质族的发展 用于分离、纯化和评估的商业价值 复杂的生物混合物。拟议的进程，尽管在这里受到限制 等离子体聚合物包覆二氧化硅和氧化铝的高效液相色谱分析 支持媒体，可以概括为包含许多不同的功能 单体，也可用于其他载体材料，包括无机 以及聚合物颗粒材料或膜。

This proposal describes the use radio frequency (RF) plasma induced polymerization for the preparation of new ion exchange support media for High Performance Liquid Chromatography (HPLC) separation of biopolymers. Silica and alumina supports will be uniformly coated with thin layers of polymerizable monomers containing ionizable groups of interest. The dispersed monomer coatings will then be polymerized on the surfaces throughout the pore structure of the supports to form thin, pin hole free chromatographic supports with a high surface density of ionizable groups. Monomers have been selected to allow preparation of both weak and strong cationic and anionic ion-exchange materials Chromatographic evaluations will be performed using proteins and other biomolecules to characterize the performance of the ion exchange media. The successful completion of this Phase I effort will lead to the evolution of families of highly selective polymer coated separations media of commercial interest for separations, purification and evaluation of complex biological mixtures. The proposed process, although limited here to the preparation of plasma polymer coated silica and alumina HPLC support media, can be generalized to incorporate many different functional monomers and can be applied to other support materials including inorganic and polymeric particulate materials or membranes.