REGULATION OF THE ANTIBODY RESPONSE TO MICROBIAL POLYSACCHARIDE ANTIGENS

脾细胞从包被的塑料皿中的结合和洗脱 单磷酰脂质A（MPL）导致> 1，000倍的富集， 当取脾细胞时，抗原特异性抑制性T细胞（Ts）活性 来自暴露于低剂量III型肺炎球菌后18-24小时的小鼠 多糖（SSS-III）。去除贴壁的Ts细胞 导致放大T细胞（TA）活性程度增加 存在于剩余的MPLS-非粘附细胞部分中;然而，两个Ts 和TA的活动中发现的MPLS-贴壁细胞部分时，脾 用最佳剂量的SSS-III免疫后4天，来自小鼠的细胞 进行了检查。这些发现以及其他发现表明，Ts和 TA -一旦激活-获得细胞表面受体，使他们能够 绑定到MPL。由于活化的动力学差异， Ts和TA在抗体应答过程中的作用以及Ts - 而TA -活性则早在暴露后18-24小时出现， SSS-III，有可能使用这种实验方法获得细胞 悬浮液大大富集Ts活性。

The binding and elution of spleen cells from plastic dishes coated with monophosphoryl lipid A (MPL) resulted in a >1,000-fold enrichment of antigen-specific suppressor T cell (Ts) activity when spleen cells taken from mice 18-24 hr after exposure to a low dose of Type Ill pneumococcal polysaccharide (SSS-III) were used. The removal of MPL-adherent Ts cells resulted in an increase in the degree of amplifier T cell (TA) activity present in the remaining MPL-nonadherent cell fraction; however, both Ts and TA activities were found in the MPL-adherent cell fraction when spleen cells from mice 4 days after immunization with an optimal dose of SSS-III were examined. These findings, as well as others, suggest that both Ts and TA -once activated - acquire a cell surface receptor that enables them to bind to MPL. Because of differences in the kinetics for the activation of Ts and TA during the course of the antibody response and the fact that Ts - but not TA - activity appears as early as 18-24 hrs after exposure to SSS-III, it is possible to use this experimental approach to obtain cell suspensions greatly enriched in Ts activity.