IMMUNOREGULATION AND IMMUNE RECOGNITION IN FILARIASIS AND NON-FILARIAL DISEASES

本项目的目的是阐明 在丝虫病和非丝虫病中调节免疫应答 states. 免疫调节研究已经检查了 抗原特异性无反应性的微丝蚴血症患者显示， 无反应性是抗增殖药物产生的结果， 细胞因子IL-10。用于主要细胞因子的过滤免疫斑测定 已经开发并用于证明蠕虫中的这一点 在人类感染中，存在Th2 CD4+细胞的扩增。 的 这些Th2 CD4+细胞的表型特征显示它们 是CD45RO + HLA-DR + CD27-;一种新的细胞内染色方法 已被用于进一步评估这些细胞因子的频率， 细胞 类似地，评估嗜酸性粒细胞活化的新方法， 也得到了发展。

The purpose of this project is to delineate the mechanisms involved in regulating immune responses in filarial and nonfilarial disease states. Immunoregulatory studies have examined the phenomenon of antigen-specific anergy in microfilaremic patients by showing this anergy to be a result of the production of the antiproliferative cytokine, IL-10. Filter immunoplaque assays for the major cytokines have been developed and used to demonstrate that in helminth infections of humans there is an expansion of Th2 CD4+ cells. The phenotypic characterization of these Th2 CD4+ cells has shown them to be CD45RO+ HLA-DR+ CD27-; a novel method for intracellular staining for cytokines has been used to assess further the frequency of these cells. Similarly, new ways of assessing eosinophil activation have also been developed.