NUCLEIC ACID BASED DETECTION AND QUANTITATION OF HIV-1 AND HIV-2

已经开发了允许检测和 通过PCR区分HIV-1和HIV-2。 当单独使用时， 引物和探针允许检测1-10个拷贝的病毒DNA。 在HIV-2临床样本中观察到超过90%的灵敏度 引物和敏感性更高的样本，从人与CD 4 少于700的。这些引物也可用于共- 具有良好灵敏度的扩增格式。 这些引物已经被用于 研究艾滋病毒和相关病毒在未知疾病中的存在， 病因如特发性CD 4 + T淋巴细胞减少症和卡波西肉瘤。 在UNG存在下，一种灵敏的RNA扩增方法是 开发通过这种方法，PCR程序的所有步骤都可以进行 在单个管中最小化携带污染。 的程序 正在开发用于原位扩增HIV DNA和RNA的方法， 通过PCR扩增和检测。 此程序正在修改， 非同位素检测系统。 这些检测方法正在使用 检测和定量唾液和血浆以及感染细胞中的病毒 从病人身上。

Primer pairs have been developed that allow the detection and discrimination of HIV-1 and HIV-2 by PCR. When used singly, these primers and probes permit detection of between 1-10 copies of viral DNA. Greater than 90% sensitivity was seen in clinical samples for the HIV-2 primers and sensitivity was greater in samples from persons with CD4 counts less than 700. These primers can also be used in a co- amplification format with good sensitivity. These primers have been used to study the presence of HIV and related viruses in diseases of unknown etiology such as Idiopathic CD4+ T-Lymphocytopenia and Kaposi's Sarcoma. A sensitive method for amplification of RNA in the presence of UNG was developed. By this method, all steps of the PCR procedure are performed in a single tube a minimizing carryover contamination. A procedure for amplification of HIV DNA and RNA is being developed for in situ amplification and detection by PCR. This procedure is being modified for non-isotopic detection systems. These detection methods are being used to detect and quantitate virus in saliva and plasma and in infected cells from patients.