MOLECULAR DEFINITION OF FILARIAL ANTIGENS

在人类丝状酵素的研究中，进展受到了1) 缺乏明确的寄生虫抗原；2)广泛的免疫学交叉- 在人类的八种丝虫物种中看到的反应性；以及3) 缺乏丰富的寄生虫材料。这个项目的目标是 定义和产生在诱导过程中重要的丝虫蛋白 寄生虫在人类宿主中的特异性免疫反应，并了解 在分子水平上，相关丝虫物种之间的差异。 CDNA库和基因组文库已被构建(Brgia Malayi，Loa Loa和Wuchereria bancrofti、螺旋体丝虫)或提供 (卷丝虫、马来丝虫幼虫cDNA文库)的筛选 有了确定的患者血清，患者T细胞或寄生虫DNA可能是 已执行。重组抗原和探针已被鉴定为a) 编码具有免疫活性和潜在保护性的班氏杆菌分子； B)可以通过限制片段区分相关的丝虫物种 长度多态、PCR或直接Southern blotting；c)识别重复 W.bancrofti基因组或Loa Loa；d)的基因组片段可以是 潜在的诊断重要性；以及e)引起即时超敏反应 淋巴丝虫病综合征的类型反应。

In the study of the human filariases, progress has been hampered by 1) the lack of defined parasite antigens; 2) the broad immunological cross- reactivity seen among the eight filarial species of humans; and 3) the dearth of abundant parasite material. The objectives of this project are to define and generate filarial proteins that are important in inducing parasite-specific immune responses in the human host and to understand, at a molecular level, the differences among related filarial species. cDNA and genomic libraries have been either constructed (Brugia malayi, Loa loa and Wuchereria bancrofti, Onchocerca volvulus) or made available (Onchocerca volvulus, Brugia malayi larval cDNA library) so that screening with defined patient sera, patient T cells or parasite DNA could be performed. Recombinant antigens and probes have been identified that a) encode immunoreactive and potentially protective molecule of W. bancrofti; b) can distinguish among related filarial species by restriction fragment length polymorphims, PCR or direct Southern blotting; c) identify repeated segments of either the W. bancrofti genome or that of Loa loa; d) may be of potential diagnostic importance; and e) induce immediate hypersensitivity type responses in lymphatic filarial syndromes.