IMMUNOREGULATION AND IMMUNE RECOGNITION IN FILARIASIS AND NON-FILARIAL DISEASES

这个项目的目的是描述所涉及的机制 在调节患者的体液和细胞反应中的作用 丝虫病和其他疾病状态。免疫调节研究 已经研究了抗原特异性无能现象 微丝状体缺乏症患者通过在 抗体(B细胞)和淋巴因子(T细胞)反应性。此外， 在活动期患者中也发现了类似的T细胞无能 盘尾丝虫病。遗传因素、抗原的影响 成分，以及对这种无反应的特异性抗丝虫病化疗 已经开始使用分子生物学工具和定义的抗原。 寄生虫抗原驱动抗体产生的体外模型 与寄生虫特异的和HTLV-1转化的T细胞克隆一样 被开发为更详细地了解这些机制 调节抗体的产生(特别是lgG和lgE) 丝虫病和非丝虫病。 血吸虫病丝虫特异性IgE和IgG的定性检测， 淋巴丝虫病和盘尾丝虫病有明确的模式 在不同的患者群体中抗原识别的不同 丝虫病的不同临床表现。使用这些 技术，由患者唯一识别的抗原 已经发现了血吸虫病或盘尾丝虫病。飞虫物种- 人源性单抗识别的特异性抗原 从EBV转化的患者中也发现了B细胞， 特色化的。此外，FPLC的分级和定性 对丝虫抗原成分的分析 发现了两组蛋白质，它们似乎调节着 细胞和体液水平的免疫反应。

The purpose of this project is to delineate the mechanisms involved in regulating the humoral and cellular responses in patients with filariaisis and other disease states. Immunoregulatory studies have examined the phenomenon of antigen-specific anergy in microfilaremic patients by showing this anergy at the level of antibody (B cell) and lymphokine (T cell) responsiveness. Further, similar T cell anergy has been found among patients with active onchocerciasis. The influence of genetic factors, antigen composition, and specific antifilarial chemotherapy on this anergy has begun using molecular biological tools and defined antigens. In vitro models of parasite-antigen driven antibody production as well as parasite-specific and HTLV-1 transformed T cell clones have been developed to understand in more detail those mechanisms regulating antibody production (particularly lgG andlgE) in filarial and non-filarial diseases. Qualitative analysis of filaria-specific lgE and lgG in loiasis, lymphatic filariasis, and onchocerciasis have indicated patterns of antigen recognition which differ among groups of patients with different clinical manifestations of filariasis. Using these techniques, antigens recognized exclusively by patients with loiasis or onchocerciasis have been identified. Fliarial species- specific antigens recognized by human monoclonal antibodies derived from EBV-transformed patient B cells have also been identified and characterized. Further, FPLC fractionation and qualitative analysis of the antigenic components of filarial parasites has identified a two sets of proteins which appears to regulate the immune response at both the cellular and humoral level.