IMMUNE STUDIES IN HOMOSEXUAL MEN AT RISK FOR ACQUIRED IMMUNE DEFICIENCY SYNDROME

来自HTLV-III抗体（+）的外周血白细胞（PBL）和 (-)检测供体的增殖（3H）和细胞介导的 流感病毒（S + X）和HLA同种异体淋巴细胞溶解（CML） 细胞（ALLO）。 在抗体（+）供体中，约50% 未能对S + X作出反应，而所有人均对ALLO作出反应，但 处于艾滋病关键阶段的患者， 免疫原的类型。 使用来自抗体（-）供体的PBL，我们通过细胞 S + X T细胞应答必须进行的分级技术 使用CD4 + T细胞，而ALLO反应可以通过 CD4+或CD4-T细胞。 因此，S + X的选择性损失 反应可能反映了艾滋病期间CD4+细胞的损失 发展 使用上述方法，我们已经测试了艾滋病中的T细胞功能 接受胸苷类似物AZT治疗的患者。 一些接受AZT治疗的患者表现出T细胞恢复， 免疫功能，但这种功能的恢复逐渐 随着时间的推移而减少。

Peripheral blood leukocytes (PBL) from HTLV-III antibody (+) and (-) donors were tested for proliferative (3H) and cell-mediated lympholysis (CML) to influenza virus (S+X) and to HLA allogeneic cells (ALLO). Among antibody (+) donors, approximately 50% failed to respond to S+X, whereas all responded to ALLO, except patients in the critical stages of AIDS, who responded to neither type of immunogen. Using PBL from antibody (-) donors we demonstrated by cell fractionation techniques that S+X T cell responses are obliged to use CD4+ T cells, whereas ALLO responses can be generated by either CD4+ or CD4- T cells. Thus, the selective loss of S+X responses probably reflects the loss of CD4+ cells during AIDS development. Using the above approach, we have tested T cell functions in AIDS patients undergoing therapy with the thymidine analog AZT. Some patients undergoing AZT exhibited a restoration of T cell immune function, but this restoration of function has gradually decreased with time.