SYNERGISTIC EFFECTS OF MURINE CYTOMEGALOVIRUS AND GRAFT-VERSUS-HOST REACTION

注射亚致死剂量小鼠巨细胞病毒(MCMV)的小鼠表现出 它们在体外产生细胞毒作用的能力迅速和戏剧性的变化 T淋巴细胞对半抗原自身和同种异体抗原的反应，并产生 IL-2和IL-2受体的表达。腹膜腔内注射后三天内 注射MCMV后，对半抗原自身和同种异体抗原的CTL反应为 废除的或严重减少的。这之后是快速恢复到 CTL电位正常水平。给F1杂交小鼠注射任何一种 MCMV或亲代脾细胞(移植物抗宿主反应-GVHR)导致 迅速而严重的免疫抑制。接种病毒或 亲本细胞的选择是为了使它们低于 严重的免疫抑制。然而，当这两种接种剂结合在一起时， 观察到严重的免疫抑制。此外，给F1小鼠注射 亲代淋巴细胞只识别I类MHC决定簇 不会导致免疫抑制。然而，I类的组合 识别和MCMV感染会导致严重的免疫抑制。 在GVH诱导前感染宿主小鼠的MCMV导致 增强免疫抑制，而供体小鼠感染MCMV 诱导前GVH导致免疫抑制降低。这个 MCMV和GVHR的结合也会导致间质性肺炎， 然而，无论是哪一种侮辱，都不会对 肺部。这些研究使研究免疫抑制成为可能。 巨细胞病毒感染与巨细胞病毒感染的可能后果 带着GVHR。

Mice injected with sublethal doses murine cytomegalovirus (MCMV) exhibit rapid and dramatic changes in their ability to generate in vitro cytotoxic T lymphocyte responses to hapten-self and to alloantigens, and to produce IL 2 and express IL 2 receptors. Within three days after intraperitoneal injection of MCMV, the CTL responses to hapten-self and alloantigens are abrogated or severely reduced. This is followed by rapid recovery to a normal level of CTL potential. Injection of F1 hybrid mice with either MCMV or parental spleen cells (graft-versus-host reaction - GVHR) resulted in rapid and severe immunosuppression. Inoculation of either the virus or parental cells were selected so that they would be below the threshold for severe immunosuppression. However, when these two inocula were combined, severe immunosuppression was observed. Furthermore, injection of F1 mice with parental lymphocytes that recognize only class I MHC determinants does not result in immune suppression. However, a combination of class I recognition and MCMV infection results in profound immune suppression. Infection of host mice with MCMV prior to induction of GVH resulted in augmented immune suppression, whereas infection of donor mice with MCMV before induction of GVH resulted in reduced immune suppression. The combination of MCMV and GVHR also resulted in interstitial pneumonitis, whereas either insult alone had no detectable pathogenic effect on the lungs. These studies permit the investigation of the immunosuppression of MCMV infection and the possibility consequences of CMV infection coupled with a GVHR.