REGIONAL CEREBRAL CIRCULATION AND METABOLISM

建立了一种定量测定这些比率的方法。 局部葡萄糖消耗在离散的功能和结构上 清醒或麻醉的实验动物的大脑的组成部分。 该方法是基于使用(14C)脱氧葡萄糖作为葡萄糖的示踪剂 通过己糖激酶步骤的助熔剂。局部[14C]脱氧葡萄糖-6-磷酸 中枢神经系统组织中的浓度是通过定量的 放射自显影方法。就像亲属的放射自显影一样 局部葡萄糖消耗率可直接用于代谢 CNS中的功能链接结构的映射，该方法正在 用于研究离散人群能量代谢的变化 大脑的各种功能和结构成分 生理、药理和病理状态。

A method has been developed for the quantitative determination of the rates of local glucose consumption in the discrete functional and structural components of the brain in conscious or anesthetized laboratory animals. The method is based on the use of (14C)deoxyglucose as a tracer for glucose flux through the hexokinase step. Local [14C]deoxyglucose-6-phosphate concentrations in the tissues of the CNS are measured by a quantitative autoradiographic method. In as much as the autoradiographs of the relative rates of local glucose consumption can be used directly for metabolic mapping of functionally linked structures in the CNS, the method is being used to study alterations in the energy metabolism of the discrete functional and structural components of the brain in a variety of physiological, pharmacological, and pathological states.