ANALYSIS OF NEUTRALIZING EPITOPES ON ROTAVIRUS OUTER CAPSID PROTEINS (VP4, VP7)

1个血清型特异性和3个血清型交叉反应的抗VP7抗体 用中和单抗(N-mAbs)研究 中和表位参与抗原性位点的形成 猪轮状病毒戈特弗里德株(血清4型)。单人、双人或 选择了三重中和抗性突变体。 这些N-单抗在体外。该病毒VP7编码基因的序列分析 突变体揭示：(I)在a处取代的氨基酸(AA) 由单个N-mAb选择的突变体VP7上的特定位置可以改变， 导致出现抗原性差异的变异； 除了可变区VR-5(AA 87-100)、VR-7(AA 141-150)、VR-8(AA 208-224)和VR-9(AA 235-242)、AA 290和291在恒定区 参与中和的VP7和(Iii)VR-5、VR-7、VR-8、 VR-9和AA 290和291在功能上相互关联。在……里面 为了进一步分析VP7上的中和点并研究 所用N-单抗之间可能的协同或拮抗作用 在这项研究中，在三种病毒存在的情况下产生了抗原变体 N-单抗。猪瘟病毒VP7编码基因的核苷酸序列分析 突变体未能表现出协同或拮抗效应 用于中和选择的单抗逃逸突变体。

One serotype-specific and three serotype-cross-reactive anti-VP7 neutralizing monoclonal antibodies (N-mAbs) were utilized to study the neutralization epitopes involved in the formation of antigenic sites on porcine rotavirus Gottfried strain (serotype 4). Single, double, or triple neutralization-resistant mutants were selected in the presence of these N-mAbs in vitro. Sequence analysis of the gene encoding VP7 of such mutants revealed that: (i) the amino acid (aa) that is substituted at a specific position on the mutant VP7 selected by a single N-mAb can vary, resulting in variants which exhibit antigenic differences; (ii) in addition to variable regions VR-5 (aa 87-100), VR-7 (aa 141-150, VR-8 (aa 208-224), and VR-9 (aa 235-242), aa 290 and 291 in the constant region of the VP7 are involved in neutralization, and (iii) VR-5, VR-7, VR-8, VR-9, and aa 290 and 291 are functionally related to one another. In order to further analyze neutralization sites on the VP7 and to study possible synergistic or antagonistic effects among the N-mAbs employed in this study, antigenic variants were generated in the presence of three N-mAbs. Nucleotide sequence analysis of the VP7-encoding gene of the mutants failed to demonstrate synergistic or antagonistic effects among the mAbs used in the selection of neutralization escape mutants.