Computer Aided Two-Dimensional Electrophoretic Gel Analysis (GELLAB)

GELLAB-II软件系统（http://www-lecb.ncifcrf.gov/lemkin/gellab.html）是一个探索性数据分析系统，用于分析2D电泳蛋白凝胶图像集。它结合了用于图像采集、处理、数据库操作、图形和统计分析的复杂子系统。它已被应用于各种实验系统，在这些实验系统中，在数百或数千个不变的蛋白质中，一个或多个蛋白质的定量和定性变化是基本的分析问题。跟踪使用这些方法检测到的更改也是系统的一个主要属性。复合凝胶数据库可以在不同的探索性数据分析条件下“查看”，并从有效的3D数据库的“切片”中阐明统计差异和微妙模式。结果可以在各种表格、图或衍生图像中呈现，并在广域网上的工作站上呈现。GELLAB-II的研究已经导致了与CSPI/Scanalytics公司的技术转让CRADA，用于商业可用的系统GELLAB-II+，使这项技术在1996年发布的廉价微软Windows pc上很容易为癌症研究人员提供。这种商业化导致了GELLAB-II技术比我们所能提供的更广泛的使用和更好的支持。Z01 BC 08381-16

The GELLAB-II software system, http://www-lecb.ncifcrf.gov/lemkin/gellab.html, is an exploratory data analysis system for the analysis of sets of 2D electrophoretic protein gel images. It incorporates sophisticated subsystems for image acquisition, processing, database manipulation, graphics and statistical analysis. It has been applied to a variety of experimental systems in which quantitative and qualitative changes, in one or more proteins among hundreds or thousands of unaltered proteins, is the basic analytic problem. Keeping track of changes detected using these methods is also a major attribute of the system. A composite gel database may be "viewed" under different exploratory data analysis conditions, and statistical differences and subtle patterns elucidated from " slices" of an effective 3D database. Results can be presented in a variety of tables, plots or derived images and on workstations over wide area networks. The GELLAB-II research has resulted in a technology transfer CRADA with CSPI/Scanalytics Inc., for a commercially available system GELLAB-II+, making this technology easily available to cancer researchers on inexpensive Microsoft Windows PCs (released in 1996). Such commercialization resulted in wider use and better support of the GELLAB-II technology than we can provide. Z01 BC 08381-16