Mass Spectrometer System

说明（由申请人提供）：申请资金用于一个质谱仪系统，该系统由ThermoFinnigan LCQ Deca XP Plus超灵敏MS n离子阱质谱仪和大气压MALDI离子源组成，配有能够进行二维色谱分析的纳升流动HPLC。该仪器系统具有高灵敏度和质量准确度以及串联质谱分析能力。它非常适合蛋白质组学应用，大分子组装的表征，以及生物标志物和技术转让应用中潜在治疗剂模式的鉴定。13个研究小组参加了这项提案，其中包括了解肥胖、减数分裂、信号转导、艾滋病毒/艾滋病、传染病和癌症机制的项目。这些仪器将由经验丰富的质谱师和蛋白质化学家在现有的共享蛋白质核心设施内操作和监督，并具有生产力和协作互动的记录。

DESCRIPTION (provided by applicant): Funds are requested for a mass spectrometer system, composed of a ThermoFinnigan LCQ Deca XP Plus ultra sensitive MS n ion trap mass spectrometer and atmospheric pressure MALDI ion source, with a nanoliter flow HPLC capable of two-dimensional chromatography. This instrument system provides high sensitivity and mass accuracy with tandem mass spectrometry capabilities. It is ideally suited for proteomics applications, characterization of macromolecular assembles, and identification of patterns of biomarkers and potential therapeutics in technology transfer applications. Thirteen groups of investigators participate in this proposal, which includes projects in understanding the mechanisms of obesity, meiosis, signal transduction, HIV/AIDS, infectious diseases and cancer. The instruments will be operated and supervised by experienced mass spectrometrists and protein chemists within an existing shared protein core facility with a record of productivity and collaborative interactions.