COBRE: LOUISVILLE RES FOUND INC: CORE B: MICROARRAY FACILITY

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。微序列阵列核心设施位于距布朗癌症中心一个街区的Baxter II生物医学研究大楼。该设施包含一个Affymetrix基因芯片仪器，可供所有MT-COBRE成员使用。该设施的组织阵列组件的开发计划也正在进行中。微序列阵列设备在沃尔夫冈·扎卡里亚斯博士的指导下，在过去三年半的时间里已经全面投入使用。它有一名全职工作人员Sabine Waigel，她在样品处理、杂交和数据分析方面提供技术援助。微序列阵列核心与病理学系的激光捕获显微镜设备和Brown癌症中心新建立的肿瘤库有着密切的关系。这样就可以制备具有已知病理特征的样品和适当的对照标本。Zacharias博士可以在实验设计和数据分析方面提供帮助。2005年3月，购买并安装了新的高分辨率Affymetrix扫描仪3000升级版。这次升级现在允许我们为所有COBRE研究人员使用最新一代高密度全基因组v2.0阵列，用于人类或小鼠。此外，还在校园里组织和举办了几次软件/数据分析讲习班，培训微阵列技术用户

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Microsequence Array Core Facility is located in the Baxter II Biomedical Research Building, one block from the Brown Cancer Center. This facility contains an Affymetrix Genechip instrument which is available to all MT-COBRE members. Plans are underway for the development of a tissue array component of this facility as well. The Microsequence Array Facility is under the direction of Dr. Wolfgang Zacharias and has been fully operational for the past three and one-half years. It contains a full-time staff member, Sabine Waigel, who provides technical assistance in sample processing, hybridization and data analysis. The Microsequence Array Core has a close relationship with the Laser Capture Microscopy facility in the Department of Pathology and a newly established Tumor Bank in the Brown Cancer Center. This allows the preparation of samples with known pathologic characteristics and appropriate control specimens. Dr. Zacharias is available for assistance with experimental design and data analysis. In March 2005, the new high-resolution Affymetrix Scanner 3000 upgrade was purchased and installed. This upgrade now allows us to use the newest generation of high-density whole genome v2.0 arrays for human or mouse for all COBRE investigators. Also, several software/data analysis workshops were organized and held on campus for training of microarray technology user