Core C: Structural Imaging Core

该提案的中心主题是剖析导致组织损伤和器官损伤的分子机制。 创伤中的功能障碍信息、蛋白质或结构变化的可视化和定位， 在这个动态过程中的每一步产生的结果是必不可少的理解的分子机制， 外伤该提案中的不同项目侧重于定义的组织系统，包括肠道，肝脏和肺 在休克或创伤模型中。在每种情况下，将采用广泛的显微镜方法， 被认为是在组织和细胞水平上理解HS病理学的关键，因此， 中央细胞和组织成像核心被定义为该提议的组成部分。核心将被安置在 在匹兹堡大学医学中心的生物成像中心（CB 1）。该中心配备 执行光学方法的连续性，包括本计划项目所必需的所有类型的显微镜。 在本计划的范围内，光学显微镜技术包括：组织学、免疫组织学、激光 共聚焦、双光子、倏逝波、活细胞和全组织/动物成像技术。我们可观的 在计算机图像处理和形态测量的经验将允许定量分析观察到的 现象，以证实微妙的质变，这是核心在这个计划中的主要功能。在 电镜水平、薄片电镜和免疫电镜评价 在需要时，将使用标本作为光学显微镜分析的自然延伸。期间 在上一个资助周期，CBl与所有项目负责人进行了广泛的合作，如 初步的数据部分，我们预计将继续扩大使用光学技术。此外我们 我们已经开发和建立了多个新的工具，以促进这些互动在各级从单一的， 整个动物的细胞 相关性（见说明）： 光学成像是程序中的一个基本核心功能，为以下各项提供基于定量图像的数据： 随后使用岩心内也可获得的计算机辅助工具进行分析

The central theme of this proposal is to dissect the molecular mechanisms leading to tissue injury, and organ dysfunction in trauma. Visualization and localization of message, protein, or structural changes leading to, or resulting from each step in this dynamic process is essential in understanding the molecular mechanisms of trauma. The different projects within this proposal focus on defined tissue systems including gut, liver and lung within models of shock and or trauma. In each case a wide range of microscopic methods will be employed and are considered essential in gaining an understanding the pathology of HS at the tissue and cellular level, thus a central cell and tissue imaging core is defined as an integral component of this proposal. The Core will be housed in the Center for Biologic Imaging (CBl) of the University of Pittsburgh Medical Center. This Center is equipped to perform a continuum of optical methods including all types of microscopy essential to this Program Project. Within the scope of this Program light microscopic techniques include: histological, immuno-histological, laser confocal, 2 photon, evanescent wave, live cell and whole dssue/animal imaging technologies. Our considerable experience in computerized image processing and morphometry will allow quantitative analysis of observed phenomena to corroborate subtle qualitative changes, and this a major function of the Core in this Program. At the electron microscopic level thin section electron microscopy and immuno-electron microscopic evaluation of specimens as a natural extension of the light microscopic analyses will be employed when needed. During the previous grant cycle the CBl collaborated extensively with all of project leaders, as is described in the preliminary data section and we expect a continued expansion in the use of optical techniques. Furthermore we have developed and built multiple new instruments to facilitate these interactions at all levels from the single cell to the whole animal RELEVANCE (See Instructions): Optical imaging is an essential core function within the program providing quantitative image based data for subsequent analysis using computer aided tools also available within the core