XENOPUS SPINDLE MATRIX PROTEOME

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 鸟苷三磷酸酶Ran（RanGTP）可以刺激V型中间丝蛋白核纤层蛋白B组装成膜性有丝分裂纺锤体基质，这是正确的有丝分裂纺锤体组装所需的。 我们在这里报告说，孤立的纺锤体矩阵包含已知的纺锤体组装因子以及信号分子和转录因子对生物体发育至关重要。有丝分裂纺锤体基质既是基于MT的纺锤体组装的张力结构，也是有丝分裂中拴系纺锤体组装因子和细胞命运决定因子的框架。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Guanosine triphosphatase Ran (RanGTP) can stimulate assembly of the type V intermediate filament protein, lamin B, into a membranous mitotic spindle matrix, which is required for proper mitotic spindle assembly. We report here that the isolated spindle matrix contains known spindle assembly factors as well as signaling molecules and transcription factors essential for organismal development. The mitotic spindle matrix is both a tensile structure for MT-based spindle assembly and a framework for tethering spindle assembly factors and cell fate determinants in mitosis.