SEMEN COLLECTION FROM MALE BABOONS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在辅助生殖技术(ART)研究中，直肠探头电射精已被证明是产生新鲜、高质量精子样本的可靠方法。为了获得用于发育生殖研究和干细胞研究的狒狒精子样本，指定的雄性狒狒通过直肠探头电射精提供精液样本进行精子分离。已确定的五个用于使用狒狒精子的领域包括： 1.通过卵泡吸引法从激素刺激的雌性狒狒采集的卵母细胞的体外受精。(精子采集程序所需的一天；种内受精) 2.非计划尸检卵巢卵母细胞的体外受精。(验尸时需要48小时分离卵母细胞的精子；种内和种间受精) 3.在获取卵子之日不能获得猕猴和黑猩猩精子的情况下，在猕猴(食蟹猴)或黑猩猩卵母细胞中进行受精研究的替代精子来源。(收集卵子所需的一天精子；异种受精) 4.精子在液氮中的冷冻保存和储存。 5.收集新鲜精子进行研究(如检测新抗体[免疫细胞化学]；生物化学研究等)。 根据当前设施标准操作程序的要求，在射精程序之间将允许被请求的巴布亚进行休息，并由兽医进行监测。对于成功产生干细胞系的男性，我们可以要求2-3个皮肤样本(~5 mm活检冲床)、3-5ml凝血管全血和/或3-5ml EDTA管内全血，用于基因分型或对男性与已建立的细胞系进行其他遗传比较。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Rectal probe electro-ejaculation of male baboons has proven reliable for producing fresh, high quality sperm samples for investigative use in assisted reproductive technologies (ART) studies. In order to obtain baboon sperm samples for developmental reproductive studies and stem cell research, assigned male baboons provided semen samples via rectal probe electro-ejaculation for sperm isolation. Five identified areas for the use of baboon sperm include: 1. In vitro fertilizations of oocytes collected via follicular aspirations from hormonally stimulated female baboons. (Sperm required day of aspiration procedures; intra-species fertilizations) 2. In vitro fertilizations of oocytes from unplanned necropsy ovaries. (Sperm required 48hr from oocyte isolations on necropsy date; intra & inter-species fertilizations) 3. Alternative sperm source for fertilization studies in either macaques (rhesus; cynomolgus) or chimpanzee oocytes when neither macaque nor chimpanzee sperm is unattainable on day of oocyte retrieval. (Sperm required day of oocyte collections; inter-species fertilizations) 4. Cryopreservation and banking of sperm in liquid nitrogen. 5. Fresh sperm collections for research investigations (i.e. testing new antibodies [immunocytochemistry]; biochemistry studies; etc). The requested baboons will be allowed rest periods between ejaculation procedures as mandated by the current facility standard operating procedure and monitored by a veterinarian. For males that successfully produce stem cell lines, we may request 2-3 skin samples (~5mm biopsy punch), 3-5mls of whole blood in clot tube and/or 3-5mls of whole blood in EDTA tube for genotyping or other genetic comparisons between the male and the established cell lines.