GLYCOPROTEINS EXPRESSING POLYSIALIC ACID AS MARKERS OF LOSS OF PLURIPOTENCY

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 干细胞计划(Dalton)已经证明，当小鼠ESC和人ESC分化为所有迄今测试的途径时，它们的表面开始表达多唾液酸(PSA)，抗PSA抗体结合证明了这一点。这一发现是由PSA合成糖基转移酶转录上调的变化提示的。该子项目的重点是确定PSA在分化过程中的表达机制及其功能。子项目的范围包括糖基因转录调控、受体蛋白表达以及确定调控机制的技术开发。综上所述，本项目展示了糖转录切割核心在指导后续实验以识别ESC分化过程中的糖链变化方面的作用。有趣的是，老鼠和人类的分化途径都显示出相似的糖链变化。这与SSEA-1和SSEA-3不同，SSEA-1和SSEA-3是在小鼠胚胎干细胞分化过程中发现的两种分化阶段特异的多糖，在人类胚胎干细胞分化中没有发现。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Stem Cell Program (Dalton) has demonstrated that both mouse ESC and human ESC, when differentiated into all pathways tested thus far, begin to express polysialic acid (PSA) on their surfaces, as evidenced by anti-PSA antibody binding. This finding was suggested by changes in PSA synthetic glycosyltransferase transcript upregulation. The Subproject focuses on determining the mechanisms of expression of PSA during differentiation and its function. The scope of the subproject encompasses glycogene transcription regulation, acceptor protein expression, and development of technologies to identify the mechanism of regulation. In sum, this project demonstrates the utility of the Glycotranscriptomics Core in directing subsequent experiments to identify glycan changes during ESC differentiation. Intriguingly, both mouse and human differentiation pathways show similar glycan changes. This is by contrast to SSEA-1 and SSEA-3, two differentiation stage-specific glycans found during mouse ESC differentiation which are not found in human ESC differentiation.