Agilent 6490 LCMS Triple Quadrupole Mass Spectrometer with 1260 Infinity Chip Cub

描述(由申请人提供)：我们正在申请资金购买一个多功能的，高灵敏度的高通量系统，由一个连接到三重四极杆质谱仪的高效液相芯片组成。该仪器将安装在北卡罗来纳大学教堂山分校(UNC-CH)环境科学与工程系的生物标记物质谱仪核心，这是一个共享设施，为整个大学和其他地方的研究人员提供服务。这种仪器将为我们提供比现有仪器至少高一个数量级的灵敏度。这将允许检测和定量分析物的水平太低，我们目前的仪器，和/或可用样品的总量排除了我们目前的仪器分析。选择的设备是安捷伦1200系列纳米流高效液相色谱仪，通过1260无限芯片立方体与安捷伦6490 LCMS三重四极杆质谱仪系统连接。这种组合是市场上最灵敏的LC-MS/MS，并将允许以最小的样品质量和体积对感兴趣的生物标志物进行定量。芯片立方体接口也非常适合在共用仪器设备中使用，在这种设备中，痕量级分析很容易受到交叉污染的影响。该设备将支持9名由NIH资助的研究人员。 与公众健康相关：生物标记物质谱仪设施为北卡罗来纳大学教堂山分校生物医学研究社区提供测量小分子和生物标记物的常规服务。为生物标记物质谱学设施申请的LC MS/MS系统将使该设施能够将复杂生物基质中小分子、DNA加合物、DNA-蛋白质交联物和其他生物标记物的分析灵敏度至少提高一个数量级。这将加强北卡罗来纳大学公共卫生学院和医学院由NIH资助的9名研究人员的研究，其中包括3名物理学家，他们研究与癌症、囊性纤维化和其他呼吸系统疾病和药物滥用有关的关键健康问题。

DESCRIPTION (provided by applicant): We are requesting funding to purchase a versatile, high sensitivity high-throughput system consisting of an HPLC-chip interfaced to a triple quadrupole mass spectrometer. This instrument will be located in the Biomarker Mass Spectrometry Facility Core in the Department of Environmental Sciences and Engineering of the University of North Carolina at Chapel Hill (UNC-CH), which is a shared facility that serves researchers from the entire university and beyond. This instrumentation will provide us with at least an order of magnitude higher sensitivity than the existing instrumentation. This will allow detection and quantitation of analytes that are present at levels too low for our current instrumentation, and/or where the total amount of sample available precludes analysis with our current instrumentation. The equipment of choice is an Agilent 1200 series nanoflow HPLC interfaced via a 1260 Infinity Chip Cube with an Agilent 6490 LCMS Triple Quadupole mass spectrometry system. This combination is the most sensitive LC-MS/MS on the market, and will allow quantitation of biomarkers of interest with the smallest mass and volume of sample. The Chip Cube interface is also very well suited for use in a shared instrument facility where trace-level analysis is very easily compromised by cross-contamination. This equipment will support nine NIH- funded researchers. PUBLIC HEALTH RELEVANCE: The Biomarker Mass Spectrometry Facility provides accessible routine service for measuring small molecules and biomarkers to the University of North Carolina at Chapel Hill biomedical research community. The LCMS/MS system requested for the Biomarker Mass Spectrometry Facility will enable the Facility to extend by at least an order of magnitude the sensitivity of assays for small molecules, DNA adducts, DNA-protein crosslinks, and other biomarkers in complex biological matrices. This will enhance studies by nine NIH-funded researchers at the UNC-CH School of Public Health and School of Medicine, including three physicianscientists, who address critical health problems related to cancer, cystic fibrosis and other respiratory disease and drug abuse.