Production of Pedigreed SPF Rhesus Macaques

 描述(申请人提供)：中国大陆维持纯种印度原产恒河猴群体已超过15年。这个起源于印度的恒河猴种群有着广泛的谱系，并且可以自我维持，不需要额外的动物招募。这项申请建议扩大印度来源的SPF恒河猴的数量和质量，提供给艾滋病资助的调查人员。动物数量和质量的扩大和提高将通过以下方式实现。1.为更小、更灵活的繁殖群体使用更小的玉米床，以扩大SPF 2级动物的生产。这将对动物生产提供更大的控制，以满足调查人员的需求。2.将CNPRC Vitals计算机程序上现有的菌落数据库转移到一个新的LabKey平台上，该平台包括电子病历和用户友好的搜索功能。3.开发改进和扩展的筛选和确认试验，以检测SPF试剂。4.将群体系谱分析从目前的微卫星平台过渡到中国遗传学和基因组学工作组(GGWC)正在开发的SNP小组。5.扩大我们对两种级别SPF动物的微生物群和免疫表型的表征，并提供独特的粪便和淋巴细胞样本生物库，这将有助于未来研究艾滋病毒与宿主微生物群和免疫系统的相互作用。纳入生物信息库核心是对一项研究的回应，该研究表明，在艾滋病相关研究中，在研究疫苗反应和治疗干预方面，了解单个动物的微生物群可能与了解其MHC单倍型一样重要。

 DESCRIPTION (provided by applicant): The CNPRC has maintained a pedigreed colony of Indian origin rhesus macaques for over 15 years. This Indian origin rhesus population has an extensive pedigree and is self-sustaining with no additional animal recruitment necessary. This application proposes to expand both the number and quality of Indian origin SPF rhesus provided to AIDS funded investigators. This expansion and improvement in animal numbers and quality will be achieved in the following way. 1. Expand production of SPF Level 2 animals by using smaller corn-cribs for smaller, more flexible breeding groups. This will provide greater control over animal production to meet investigator needs. 2. Transfer the current colony database on the CNPRC Vitals computer program to a new LabKey platform that includes electronic medical records as well as a user friendly search function. 3. Develop improved and expanded screening and confirmatory tests for detection of SPF agents. 4. Transition the colony pedigree analysis from the current microsatellite platform to the SNP panel being developed by the Genetics and Genomics Working Group (GGWC) Consortium of the CNPRC. 5. Expand our characterization of the microbiome and immune phenotype of both levels of SPF animals and provide a unique biorepository of fecal and lymphocyte samples which will facilitate future research projects investigating the interaction of HIV with the host microbiome and immune system. The inclusion of the Biorepository Core is in response to research that suggests that knowledge of an individual animal's microbiome may be as important as understanding its MHC haplotype in studying both vaccine response and therapeutic intervention in AIDS related studies.