JHU Center for Neuroscience Research

JHU Center for Neuroscience Research神经科学研究中心 在我们的“JHU神经科学研究中心”NINDS P30中心赠款的这一修订后的竞争性更新中， 我们建议保留和扩建约翰霍普金斯大学的两个科学核心设施 医学院（JHU SOM）在过去的10年里一直致力于该中心：多光子 在一些实施方案中，ES细胞工程化核心（ES Cell Engineering Core，ES细胞工程化核心）包括小鼠胚胎干细胞成像（MPI）核心和小鼠诱变核心（MMC;正式名称为“ES细胞工程化核心”）。这些 科学核心目前提供关键的研究能力和资源， 神经科学研究人员自己提供，但大大提高了个人研究的努力 程序.这些核心所提供的实验机会和技术服务是互补的， 不重复，其他设施提供给NINDS资助的调查人员在JHU SOM。中心核心的使用将 继续受益于12个NINDS资助的研究项目，这些项目构成了主要中心研究者， 这个中心，以及其他NINDS资助和非NINDS资助的研究人员在JHU SOM。研究 主要中心研究者的项目解决了一系列重要的未解决的问题， NINDS使命。这些包括对兴奋性突触动力学的基础神经科学研究， 功能，海马位置细胞回路的组织，皮层回路的定义， 躯体感觉，成人神经发生过程中局部回路的建立，细胞和分子 疼痛和瘙痒的机制，以及Na通道调节中的Ca++信号。临床相关工作 包括中风、帕金森氏病、肌萎缩侧索硬化症、 硬化症、自闭症谱系障碍和睡眠。中心核心的独特能力将继续服务于 作为NINDS资助的JHU研究人员的重要资源， 并作为传播和实际培训最新技术的协调中心 研究方法。通过这些努力，JHU神经科学研究中心将帮助建立 研究健康和疾病中大脑功能的新策略，将有利于更广泛的神经科学 社区

SUMMARY: JHU Center for Neuroscience Research In this revised competitive renewal of our “JHU Center for Neuroscience Research” NINDS P30 center grant, we propose to both maintain and expand two of the Scientific Core facilities at the Johns Hopkins University School of Medicine (JHU SOM) that have anchored this Center for the past 10 years: the Multiphoton Imaging (MPI) Core and the Murine Mutagenesis Core (MMC; formally “ES Cell Engineering Core”). These Scientific Cores currently provide critical research capabilities and resources that are impractical for neuroscience investigators to provide on their own but that greatly enhance efforts of individual research programs. The experimental opportunities and technical services offered by these Cores complement, but do not duplicate, other facilities available to NINDS-funded investigators at JHU SOM. Use of Center Cores will continue to benefit the 12 NINDS-funded research programs that constitute the Primary Center Investigators of this Center, and also other NINDS-funded and non-NINDS-funded investigators at JHU SOM. The research programs of the Primary Center Investigators address a wide range of significant unsolved questions central to the NINDS mission. These include basic neuroscience investigation into excitatory synapse dynamics and function, the organization of hippocampal place cell circuitry, definition of cortical circuits underlying somatosensation, establishment of local circuitry during adult neurogenesis, cellular and molecular mechanisms of pain and itch, and Ca++ signaling in Na channel regulation. Clinically relevant work encompasses the genetics and molecular underpinnings of stroke, Parkinson's Disease, amyotrophic lateral sclerosis, autism spectrum disorders, and sleep. The unique capabilities of Center Cores will continue to serve as a crucial resource for NINDS-funded investigators at JHU by extending the research capabilities of individual investigators and by serving as a focal point for dissemination and practical training in state-of-the art research approaches. Through these efforts, the JHU Center for Neuroscience Research will help establish new strategies for studying brain function in health and disease that will benefit the broader neuroscience community.