Acquisition of an in vivo imaging system

自从第一台显微镜问世以来，获得细胞、组织和整个动物的图像的能力一直处于生物技术进步的前沿，并导致了包括光学成像在内的各种成像方式的发展。生物发光和近红外(NIR)荧光光学成像可以可视化各种细胞过程，并且已经从从单个时间点观察组织之间的解剖差异发展到在同一动物或植物(幼苗)中以非侵入性的方式纵向成像多种生物特征。直到最近，活体成像还需要非常昂贵(通常为25万美元)的仪器，而这些仪器的操作也相对复杂。相比之下，所要求的新的珍珠三部曲活体成像系统价格实惠，操作简单。这种生物发光和近红外体内成像系统提供了足够的目标成像选项，以满足我们实验室中绝大多数(&gt；95%)的实验需求。我们申请资金购买这台仪器，否则这台仪器在舍布鲁克大学的主校区是买不到的，以支持我们在许多领域使用小型动植物进行活体成像的研究。这台仪器对我们的研究项目至关重要，在成像平台协调员的监督下，它将支持许多新项目，并有助于培训来自几个实验室的高素质人员。

The ability to obtain images of cells, tissues, and whole animals has been at the forefront of biological technological advance since the advent of the first microscope and has resulted in the evolution of various imaging modalities, including optical imaging. Bioluminescent and near-infrared (NIR) fluorescent optical imaging allows the visualization of various cellular processes and has evolved from observing anatomical differences between tissues from a single time point to imaging multiple biological features longitudinally in a noninvasive manner in the same animal or plant (seedling). Until recently, in vivo imaging required very expensive (often >$250,000) instruments that were also relatively complex to operate. In contrast, the requested new Pearl Trilogy in vivo imaging system is affordable and simple to run. This bioluminescent and NIR in vivo imaging system delivers enough targets imaging options for the vast majority (>95%) of experimental needs in our laboratories. We request funding to purchase this instrument, that is otherwise unavailable on the main campus of the Université de Sherbrooke, to support our research in many fields using small animals and plants in vivo imaging. This instrument is essential for our research programs, and will, under the supervision of a imaging platform coordinator, enable many new projects as well as contribute to the training of highly qualified personnel from several laboratories.