EFRI-BSBA: Photonic Technique for Sensing and Understanding Subcellular Structures at Nanoscale

EFRI-BSBA:用于传感和理解纳米级亚细胞结构的光子技术

基本信息

  • 批准号:
    0937987
  • 负责人:
  • 金额:
    $ 200万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2009
  • 资助国家:
    美国
  • 起止时间:
    2009-09-01 至 2014-08-31
  • 项目状态:
    已结题

项目摘要

ABSTRACT Photonic technique for sensing and understanding subcellular structures at nanoscale PI: Vadim Backman This project is at the interface of biophotonics, macromolecular biophysics, biology and medicine. The main focus of the project is the development of a photonics technique for sensing the complexity of cellular structure at the nanoscale and its use for understanding alterations in cell nanoarchitecture in early carcinogenesis with the potential impact to advance fundamental knowledge of the initial stage of carcinogenesis and enable, for the first time, population-wide screening for a wide range of major cancers. The project addresses the following two BSBA elements: i) "paradigm shift in practices and applications in engineering, health care, medicine, and biological research" and ii) "sensing at the nanoscale, novel photonic techniques for detecting, identifying, characterizing, and understanding subcellular structures and processes at nanoscale". The applicants have developed a novel optical technique, single-cell partial-wave spectroscopy (PWS), which quantifies the statistical properties of nanoscale cellular structure. Using PWS, we demonstrated that an increase in the disorder of cell nanoarchitecture is one of the earliest events in carcinogenesis which precedes any known microscale alterations. Importantly, the increased nanoscale disorder is not restricted to tumor cells but seen diffusely throughout the organ thus serving as a marker of field carcinogenesis. This provides the impetus to elucidate the mechanisms that determine these nanoscale changes and their role in cancer progression, which is the main goal of this project. Using PWS the following three key questions will be adressed: i) Is the increase in the disorder of cell nanoarchitecture a general phenomenon that takes place in essentially all types of epithelial cancers? ii) What is the origin of these changes? iii) What are the biological implications and possible role in carcinogenesis of the nanoscale disorder? In other words, is this alteration a "side-effect" of other genetic events or is it a necessary step in carcinogenesis? Although it is well accepted that early cancer screening would dramatically decrease cancer mortality, no test currently exists for accurate screening of the most lethal cancers. This is largely because the current state-of-the-art requires direct examination of an already formed precancerous lesion through interventional procedures such as colonoscopy, endoscopy, bronchoscopy, etc. These procedures are expensive, laborious, invasive and not well tolerated by patients, leading to very poor screening rates. This project may lead to a new general paradigm of cancer screening where the presence of neoplasia is detected by the non-invasive optical analysis of non-neoplastic tissue that might be located at a distance from the neoplastic focus in an easily accessible part of the organ. The vision is that this methodology may enable screening for the major types of cancer, for example, during an annual physical exam. Examples include identifying patients with colonic adenomas by analysis of rectal cells, lung cancer by analysis of buccal cells, pancreatic cancer by analysis of duodenal cells, ovarian cancer through analysis of uterine or cervical cells and esophageal adenocarcinoma through analysis of upper-esophageal squamous mucosa. From the educational perspective, the project will provide opportunities in truly interdisciplinary research at the interface of biophotonics, molecular biophysics and cancer biology for graduate, undergraduate and high school students with a particular focus on recruitment of women and minorities in science. The findings will be incorporated into two graduate classes directed by the PI's. The findings and data generated during the course of the project will be disseminated to the scientific community. The main findings will also be disseminated to the general public.
摘要纳米级光子学传感和理解亚细胞结构的研究这个项目是生物光子学、大分子生物物理学、生物学和医学的交界点。该项目的主要重点是开发一种光子学技术,用于在纳米尺度上感知细胞结构的复杂性,并将其用于了解早期癌症发生过程中细胞纳米结构的变化,这可能会促进对癌症发生初期的基础知识,并首次能够对广泛的主要癌症进行全人群筛查。该项目涉及以下两个BSBA要素:i)“工程、保健、医学和生物研究中实践和应用的范式转变”和ii)“纳米尺度的传感,用于探测、识别、表征和理解纳米尺度的亚细胞结构和过程的新的光子技术”。申请人开发了一种新的光学技术,单细胞部分波谱(PWS),它量化了纳米细胞结构的统计特性。利用PWS,我们证明了细胞纳米结构紊乱的增加是致癌过程中最早的事件之一,发生在任何已知的微尺度变化之前。重要的是,这种增加的纳米级无序并不局限于肿瘤细胞,而是弥漫在整个器官中,因此可以作为现场致癌的标志。这为阐明决定这些纳米级变化的机制及其在癌症进展中的作用提供了动力,这是该项目的主要目标。使用PWS将涉及以下三个关键问题:i)细胞纳米结构紊乱的增加是基本上在所有类型的上皮性癌症中发生的普遍现象吗?二)这些变化的起因是什么?3)这种纳米级紊乱的生物学含义和可能的致癌作用是什么?换句话说,这种改变是其他基因事件的“副作用”,还是致癌过程中的必要步骤?虽然人们普遍认为,早期癌症筛查将显著降低癌症死亡率,但目前还没有对最致命的癌症进行准确筛查的测试。这在很大程度上是因为目前最先进的技术需要通过介入性程序(如结肠镜、内窥镜、支气管镜等)直接检查已经形成的癌前病变。这些程序昂贵、费力、有创,患者不能很好地耐受,导致筛查率非常低。这一项目可能导致癌症筛查的新的一般范例,其中肿瘤的存在是通过对非肿瘤组织的非侵入性光学分析来检测的,非肿瘤组织可能位于器官中容易接近的部位,与肿瘤焦点相距较远。我们的愿景是,这种方法可以实现对主要类型癌症的筛查,例如,在年度体检期间。例如,通过直肠细胞分析识别结肠腺瘤患者,通过口腔细胞分析识别肺癌,通过十二指肠细胞分析识别胰腺癌,通过子宫或宫颈细胞分析识别卵巢癌,通过食道上段鳞状黏膜分析识别食管腺癌。从教育的角度来看,该项目将为研究生、本科生和高中生提供生物光子学、分子生物物理学和癌症生物学之间真正跨学科研究的机会,特别侧重于招募妇女和少数群体从事科学研究。研究结果将被纳入两个研究生班,由国际和平研究所指导。研究结果和在项目过程中产生的数据将传播给科学界。主要研究结果亦会向公众公布。

项目成果

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Vadim Backman其他文献

Sa1712 Differential Effects of Tumor-Promoting and Tumor-Inhibiting Dietary Fats on Angiogenesis in Normal and Tumor-Bearing Colon
  • DOI:
    10.1016/s0016-5085(13)61025-2
  • 发表时间:
    2013-05-01
  • 期刊:
  • 影响因子:
  • 作者:
    Vani J. Konda;Mariano Gonzalez-Haba Ruiz;Sarah Ruderman;Vesta Valuckaite;Urszula Dougherty;Reba Mustafi;Anirudh Kulkarni;Tiffany Chua;Irving Waxman;Vadim Backman;John Hart;Marc Bissonnette
  • 通讯作者:
    Marc Bissonnette
Influence of Micropillar Induced Deformation on Chromatin Architecture in Regulating Stem Cell Differentiation
  • DOI:
    10.1016/j.bpj.2019.11.2999
  • 发表时间:
    2020-02-07
  • 期刊:
  • 影响因子:
  • 作者:
    Vasundhara Agrawal;Xinlong Wang;Guillermo Ameer;Vadim Backman
  • 通讯作者:
    Vadim Backman
Differential unfolded protein response regulation in KRAS silencing sensitive and innately resistant colorectal cancer cells
KRAS 沉默敏感和先天耐药结直肠癌细胞中差异展开的蛋白反应调节
  • DOI:
    10.1038/s41598-025-94549-2
  • 发表时间:
    2025-04-24
  • 期刊:
  • 影响因子:
    3.900
  • 作者:
    Flávia Martins;Ana L. Machado;Joana Carvalho;Catarina R. Almeida;Hans C. Beck;Ana S. Carvalho;Vadim Backman;Rune Matthiesen;Sérgia Velho
  • 通讯作者:
    Sérgia Velho
Bridging Chromatin Nanoimaging and Molecular Modeling: Chromatin Packing as a Regulator of Transcriptional Heterogeneity in Carcinogenesis
  • DOI:
    10.1016/j.bpj.2019.11.3007
  • 发表时间:
    2020-02-07
  • 期刊:
  • 影响因子:
  • 作者:
    Vadim Backman
  • 通讯作者:
    Vadim Backman
Large-Scale Heteropolymer Model of Chromatin Dynamics and Mechanics
  • DOI:
    10.1016/j.bpj.2019.11.3011
  • 发表时间:
    2020-02-07
  • 期刊:
  • 影响因子:
  • 作者:
    Anne Shim;Kai Huang;Vadim Backman;Igal Szleifer
  • 通讯作者:
    Igal Szleifer

Vadim Backman的其他文献

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{{ truncateString('Vadim Backman', 18)}}的其他基金

EFRI CEE: Macrogenomic engineering via modulation of chromatin nanoenvironment
EFRI CEE:通过调节染色质纳米环境进行宏观基因组工程
  • 批准号:
    1830961
  • 财政年份:
    2018
  • 资助金额:
    $ 200万
  • 项目类别:
    Standard Grant
EFRI-BioFlex: Miniature, low-cost fiber-optics technology for measurement of tissue structure at sub-diffractional length scales: a platform for cancer screening
EFRI-BioFlex:用于在亚衍射长度尺度上测量组织结构的微型、低成本光纤技术:癌症筛查平台
  • 批准号:
    1240416
  • 财政年份:
    2012
  • 资助金额:
    $ 200万
  • 项目类别:
    Standard Grant
EAGER: New Biophotonics and Computational Molecular Dynamics for Understanding Epigenetic Regulation of Gene Transcription
EAGER:新生物光子学和计算分子动力学用于理解基因转录的表观遗传调控
  • 批准号:
    1249311
  • 财政年份:
    2012
  • 资助金额:
    $ 200万
  • 项目类别:
    Standard Grant
MRI-R2: Development of biophotonics instrumentation for sensing subcellular structure at nanoscale
MRI-R2:开发用于传感纳米级亚细胞结构的生物光子学仪器
  • 批准号:
    0960148
  • 财政年份:
    2010
  • 资助金额:
    $ 200万
  • 项目类别:
    Standard Grant
EAGER: BISH: Biophotonics Technique for Detection of Lung Cancer
EAGER:BISH:用于检测肺癌的生物光子学技术
  • 批准号:
    0939778
  • 财政年份:
    2009
  • 资助金额:
    $ 200万
  • 项目类别:
    Standard Grant
SGER: Biophotonics for Diagnosis of Pancreatic Cancer
SGER:用于诊断胰腺癌的生物光子学
  • 批准号:
    0733868
  • 财政年份:
    2007
  • 资助金额:
    $ 200万
  • 项目类别:
    Standard Grant
SGER: Biophotonics Techniques for Accurate Diagnosis of Pancreatic Cancer
SGER:准确诊断胰腺癌的生物光子学技术
  • 批准号:
    0620303
  • 财政年份:
    2006
  • 资助金额:
    $ 200万
  • 项目类别:
    Standard Grant
Biophotonics: Towards Nondestructive Optical Analysis of Nanostructures Using Photonic Nanojets
生物光子学:利用光子纳米喷射对纳米结构进行无损光学分析
  • 批准号:
    0522639
  • 财政年份:
    2005
  • 资助金额:
    $ 200万
  • 项目类别:
    Standard Grant
SGER: Noninvasive Detection of Pancreatic Cancer Using Biophotonics Techniques
SGER:利用生物光子技术无创检测胰腺癌
  • 批准号:
    0547480
  • 财政年份:
    2005
  • 资助金额:
    $ 200万
  • 项目类别:
    Standard Grant
SGER: Development of Minimally Invasive Optical Technique for Early Detection of Pancreatic Cancer
SGER:开发用于早期检测胰腺癌的微创光学技术
  • 批准号:
    0417689
  • 财政年份:
    2004
  • 资助金额:
    $ 200万
  • 项目类别:
    Standard Grant

相似海外基金

US-Japan Workshop on Bioinspired Sensing and Bioinspired Actuation (BSBA) Technologies; Hawaii; March 18 and 19, 2011
美日仿生传感和仿生驱动 (BSBA) 技术研讨会;
  • 批准号:
    1112579
  • 财政年份:
    2011
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    $ 200万
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EFRI BSBA: Complex microsystem networks inspired by internal insect physiology
EFRI BSBA:受昆虫内部生理学启发的复杂微系统网络
  • 批准号:
    0938047
  • 财政年份:
    2010
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    $ 200万
  • 项目类别:
    Standard Grant
EFRI-BSBA: Biology Inspired Intelligent Micro Optical Imaging Systems
EFRI-BSBA:受生物学启发的智能微光学成像系统
  • 批准号:
    0937847
  • 财政年份:
    2009
  • 资助金额:
    $ 200万
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    Standard Grant
EFRI-BSBA: Learning from Plants -- Biologically-Inspired Multi-Functional Adaptive Structural Systems
EFRI-BSBA:向植物学习——受生物启发的多功能自适应结构系统
  • 批准号:
    0937323
  • 财政年份:
    2009
  • 资助金额:
    $ 200万
  • 项目类别:
    Standard Grant
EFRI-BSBA Integration of Dynamic Sensing and Actuating of Neural Microcircuits
EFRI-BSBA 动态传感与神经微电路驱动的集成
  • 批准号:
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  • 财政年份:
    2009
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    $ 200万
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EFRI-BSBA: Engineering Synthetic Mimics of DNA-Protein Recognition Systems
EFRI-BSBA:DNA-蛋白质识别系统的工程合成模拟
  • 批准号:
    0938019
  • 财政年份:
    2009
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    $ 200万
  • 项目类别:
    Standard Grant
EFRI-BSBA: Nanoactuation and Sensing of Neural Function for Engineering Future Biomimetic Retinal Implants and Therapies
EFRI-BSBA:神经功能的纳米驱动和传感,用于工程未来仿生视网膜植入物和治疗
  • 批准号:
    0938072
  • 财政年份:
    2009
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    $ 200万
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EFRI- BSBA: Novel Microsystems for Manipulation and Analysis of Immune Cells
EFRI- BSBA:用于免疫细胞操作和分析的新型微系统
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  • 财政年份:
    2009
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    $ 200万
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EFRI-BSBA: Second Window
EFRI-BSBA:第二个窗口
  • 批准号:
    0937710
  • 财政年份:
    2009
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    $ 200万
  • 项目类别:
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EFRI-BSBA: Multifunctional Materials and Devices for Distributed Actuation and Sensing
EFRI-BSBA:用于分布式驱动和传感的多功能材料和设备
  • 批准号:
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  • 资助金额:
    $ 200万
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